Bacteroides thetaiotaomicron Starch Utilization Promotes Quercetin Degradation and Butyrate Production by Eubacterium ramulus

被引:43
作者
Paola Rodriguez-Castano, Gina [1 ]
Dorris, Matthew R. [2 ]
Liu, Xingbo [2 ]
Bolling, Bradley W. [2 ]
Acosta-Gonzalez, Alejandro [1 ]
Rey, Federico E. [3 ]
机构
[1] Univ La Sabana, Fac Ingn, Grp Invest Bioprospecc GIBP, Chia, Colombia
[2] Univ Wisconsin, Dept Food Sci, 1605 Linden Dr, Madison, WI 53706 USA
[3] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
基金
美国食品与农业研究所;
关键词
quercetin degradation; butyrate; Eubacterium ramulus; Bacteroides thetaiotaomicron; cross-feeding; starch; POLYSACCHARIDE UTILIZATION; ANAEROBIC DEGRADATION; RESISTANT STARCH; FECAL SAMPLES; FLAVONOIDS; BACTERIA; QUANTIFICATION; FERMENTATION; METABOLISM; GLYCOSIDES;
D O I
10.3389/fmicb.2019.01145
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Consumption of flavonoids has been associated with protection against cardiovascular and neurodegenerative diseases. Most dietary flavonoids are subjected to bacterial transformations in the gut where they are converted into biologically active metabolites that are more bioavailable and have distinct effects relative to the parent compounds. While some of the pathways involved in the breakdown of flavonoids are emerging, little it is known about the impact of carbon source availability and community dynamics on flavonoid metabolism. This is relevant in the gut where there is a fierce competition for nutrients. In this study, we show that metabolism of one of the most commonly consumed flavonoids, quercetin, by the gut-associated bacterium Eubacterium ramulus is dependent on interspecies cross-feeding interactions when starch is the only energy source available. E. ramulus can degrade quercetin in the presence of glucose but is unable to use starch for growth or quercetin degradation. However, the starch-metabolizing bacterium Bacteroides thetaiotaomicron, which does not metabolize quercetin, stimulates degradation of quercetin and butyrate production by E. ramulus via cross-feeding of glucose and maltose molecules released from starch. These results suggest that dietary substrates and interactions between species modulate the degradation of flavonoids and production of butyrate, thus shaping their bioavailability and bioactivity, and likely impacting their health-promoting effects in humans.
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页数:8
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共 43 条
  • [1] Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut
    Belenguer, Alvaro
    Duncan, Sylvia H.
    Calder, A. Graham
    Holtrop, Grietje
    Louis, Petra
    Lobley, Gerald E.
    Flint, Harry J.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (05) : 3593 - 3599
  • [2] Degradation of quercetin and luteolin by Eubacterium ramulus
    Braune, A
    Gütschow, M
    Engst, W
    Blaut, M
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (12) : 5558 - 5567
  • [3] Pharmacokinetics and modeling of quercetin and metabolites
    Chen, X
    Yin, OQP
    Zuo, Z
    Chow, MSS
    [J]. PHARMACEUTICAL RESEARCH, 2005, 22 (06) : 892 - 901
  • [4] MEASUREMENT OF STARCH FERMENTATION IN THE HUMAN LARGE-INTESTINE
    CUMMINGS, JH
    ENGLYST, HN
    [J]. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1991, 69 (01) : 121 - 129
  • [5] Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis
    Flint, Harry J.
    Bayer, Edward A.
    Rincon, Marco T.
    Lamed, Raphael
    White, Bryan A.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2008, 6 (02) : 121 - 131
  • [6] Of the major phenolic acids formed during human microbial fermentation of tea, citrus, and soy flavonoid supplements, only 3,4-dihydroxyphenylacetic acid has antiproliferative activity
    Gao, K
    Xu, AL
    Krul, C
    Venema, K
    Liu, Y
    Niu, YT
    Lu, JX
    Bensoussan, L
    Seeram, NP
    Heber, D
    Henning, SM
    [J]. JOURNAL OF NUTRITION, 2006, 136 (01) : 52 - 57
  • [7] Gao ZG, 2009, DIABETES, V58, P1509, DOI 10.2337/db08-1637
  • [8] DIETARY ANTIOXIDANT FLAVONOIDS AND RISK OF CORONARY HEART-DISEASE - THE ZUTPHEN ELDERLY STUDY
    HERTOG, MGL
    FESKENS, EJM
    HOLLMAN, PCH
    KATAN, MB
    KROMHOUT, D
    [J]. LANCET, 1993, 342 (8878) : 1007 - 1011
  • [9] CONTENT OF POTENTIALLY ANTICARCINOGENIC FLAVONOIDS OF 28 VEGETABLES AND 9 FRUITS COMMONLY CONSUMED IN THE NETHERLANDS
    HERTOG, MGL
    HOLLMAN, PCH
    KATAN, MB
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (12) : 2379 - 2383
  • [10] The sugar moiety is a major determinant of the absorption of dietary flavonoid glycosides in man
    Hollman, PCH
    Bijsman, MNCP
    van Gameren, Y
    Cnossen, EPJ
    de Vries, JHM
    Katan, MB
    [J]. FREE RADICAL RESEARCH, 1999, 31 (06) : 569 - 573