Effects of two oils and 16 pure polycyclic aromatic hydrocarbons on plasmatic immune parameters in the European sea bass, Dicentrarchus labrax (Linne)

被引:25
作者
Bado-Nilles, A. [1 ,2 ]
Quentel, C. [3 ]
Thomas-Guyon, H. [2 ]
Le Floch, S. [1 ]
机构
[1] Cedre, Ctr Documentat Rech & Expt Pollut Accidentelles E, F-29218 Brest 2, France
[2] Univ La Rochelle, CNRS, LIENSs Littoral Environm & Soc, UMR 6250, F-17000 La Rochelle, France
[3] Agence Francaise Securite Sanit Aliments, F-29280 Plouzane, France
关键词
Lysozyme; Complement activity; Spectrophotometry; European sea bass; Direct PAH toxicity; Field concentrations; High concentrations; DIOXIN-LIKE; CELL-LINE; PARTICLES; RESPONSES; TOXICITY; EXPOSURE;
D O I
10.1016/j.tiv.2008.12.001
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on two plasmatic immune parameters, lysozyme concentration and haemolytic alternative complement activity, of the European sea bass, Dicentrarchus labrax, were tested using field (10(-7) and 10(-9) mg mL(-1)) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. Peripheral blood from 105 fish was collected, centrifuged at 1200g, for 10 min, at 4 degrees C and three plasma pools, each of 35 fish, were constituted. Two oils (heavy fuel oil and light cycle oil) and 16 pure PAHs, selected on the basis of the American Environmental Protection Agency list (US EPA), were tested in vitro on the two humoral immune parameters. Only three pure PAHs (anthracene, chrysene and dibenz[a,h]anthracene) modulated lysozyme concentration. Acenaphthene, acenaphthylene, anthracene, benzo[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, pyrene and light cycle oil modified the haemolytic alternative complement activity after 4 h of incubation. This study investigates the direct effects of several PAHs on fish humoral immune functions and describes the haemolytic complement activity of fish as suitable biomarkers of oil pollution. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:235 / 241
页数:7
相关论文
共 42 条
[1]   Sublethal toxicity of the Prestige oil spill on yellow-legged gulls [J].
Alonso-Alvarez, Carlos ;
Munilla, Ignacio ;
Lopez-Alonso, Marta ;
Velando, Alberto .
ENVIRONMENT INTERNATIONAL, 2007, 33 (06) :773-781
[2]  
ANDERSON JW, 1974, MAR BIOL, P75
[3]   Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg) [J].
Bado-Nilles, Anne ;
Gagnaire, Beatrice ;
Thomas-Guyon, Helene ;
Le Floch, Stephane ;
Renault, Tristan .
TOXICOLOGY IN VITRO, 2008, 22 (06) :1610-1617
[4]  
BADONILLES A, COMMUNICATION
[5]   Effect of linear alkylbenzene sulphonate (LAS) on non-specific defence mechanisms in rainbow trout (Oncorhynchus mykiss) [J].
Bakirel, T ;
Keles, O ;
Karatas, S ;
Özcan, M ;
Türkmen, G ;
Candan, A .
AQUATIC TOXICOLOGY, 2005, 71 (02) :175-181
[6]   Brominated dioxin-like compounds: in vitro assessment in comparison to classical dioxin-like compounds and other polyaromatic compounds [J].
Behnisch, PA ;
Hosoe, K ;
Sakai, S .
ENVIRONMENT INTERNATIONAL, 2003, 29 (06) :861-877
[7]  
BILLIARD SM, 2004, ECOTOXICOLOGY ENV SA, P292
[8]  
BOEHM PD, 2007, MARINE POLLUTION B, P339
[9]  
BOLS NC, 1999, ECOTOXICOLOGY ENV SA, P118
[10]   Haematological, biochemical and immunological parameters as stress indicators in Dicentrarchus labrax and Sparus aurata farmed in off-shore cages [J].
Caruso, G ;
Genovese, L ;
Maricchiolo, G ;
Modica, A .
AQUACULTURE INTERNATIONAL, 2005, 13 (1-2) :67-73