Nitrite reductase of Nitrosomonas europaea is not essential for production of gaseous nitrogen oxides and confers tolerance to nitrite

被引：105
作者：
Beaumont, HJE
Hommes, NG
Sayavedra-Soto, LA
Arp, DJ
Arciero, DM
Hooper, AB
Westerhoff, HV
van Spanning, RJM
机构：
[1] Free Univ Amsterdam, Fac Biol, Dept Mol Cell Physiol, BioCtr Amsterdam, NL-1081 HV Amsterdam, Netherlands
[2] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA
[3] Univ Minnesota, Dept Biochem Mol Biol & Biophys, St Paul, MN 55108 USA
来源：
JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 09期
关键词：
D O I：
10.1128/JB.184.9.2557-2560.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A gene that encodes a periplasmic copper-type nitrite reductase (NirK) was identified in Nitrosomonas europaea. Disruption of this gene resulted in the disappearance of Nir activity in cell extracts. The nitrite tolerance of NirK-deficient cells was lower than that of wild-type cells. Unexpectedly, NirK-deficient cells still produced nitric oxide (NO) and nitrous oxide (N2O), the latter in greater amounts than that of wild-type cells. This demonstrates that NirK is not essential for the production of NO and N2O by N. europaea. Inactivation of the putative fnr gene showed that Fnr is not essential for the expression of nirK.
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收藏
页码：2557 / +
页数：5
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