Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells

被引:228
作者
Choi, Chul Hee [1 ]
Lee, Jun Sik [1 ]
Lee, Yoo Chul [1 ]
Park, Tae In [2 ]
Lee, Je Chul [1 ]
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Microbiol, Taegu 700422, South Korea
[2] Kyungpook Natl Univ, Sch Med, Dept Pathol, Taegu 700422, South Korea
关键词
D O I
10.1186/1471-2180-8-216
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Acinetobacter baumannii is a nosocomial pathogen of increasing importance, but the pathogenic mechanism of this microorganism has not been fully explored. This study investigated the potential of A. baumannii to invade epithelial cells and determined the role of A. baumannii outer membrane protein A (AbOmpA) in interactions with epithelial cells. Results: A. baumannii invaded epithelial cells by a zipper-like mechanism, which is associated with microfilament- and microtubule-dependent uptake mechanisms. Internalized bacteria were located in the membrane-bound vacuoles. Pretreatment of recombinant AbOmpA significantly inhibited the adherence to and invasion of A. baumannii in epithelial cells. Cell invasion of isogenic AbOmpA-mutant significantly decreased as compared with wild-type bacteria. In a murine pneumonia model, wild-type bacteria exhibited a severe lung pathology and induced a high bacterial burden in blood, whereas AbOmpA-mutant was rarely detected in blood. Conclusion: A. baumannii adheres to and invades epithelial cells. AbOmpA plays a major role in the interactions with epithelial cells. These findings contribute to the understanding of A. baumannii pathogenesis in the early stage of bacterial infection.
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页数:11
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