Hydrogenases: active site puzzles and progress

被引：168

作者：

Armstrong, FA ^{[1
]}

机构：

[1] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2004年 / 8卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.cbpa.2004.02.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent research on the hydrogenase reactions has sought to probe beyond the information that is provided by X-ray diffraction structures. The major challenge of locating 'transient' hydrogen atoms in species that are potential catalytic intermediates is being addressed, using advanced electron paramagnetic resonance (EPR) techniques and theoretical methods. This article discusses recent progress towards a consensus on the structures of different states of the active site of hydrogenases, the mechanisms of activation and hydrogen cycling.

引用

页码：133 / 140

页数：8

共 46 条

[1] The [NiFe] hydrogenase from Allochromatium vinosum studied in EPR-detectable states:: H/D exchange experiments that yield new information about the structure of the active site [J].

Bleijlevens, B ;

Faber, BW ;

Albracht, SPJ .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2001, 6 (08) :763-769

[2] Metal insertion into NiFe-hydrogenases [J].

Blokesch, M ;

Paschos, A ;

Theodoratou, E ;

Bauer, A ;

Hube, M ;

Huth, S ;

Böck, A .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :674-680

[3] Direct detection of a hydrogen ligand in the [NiFe] center of the regulatory H2-sensing hydrogenase from Ralstonia eutropha in its reduced state by HYSCORE and ENDOR spectroscopy [J].

Brecht, M ;

van Gastel, M ;

Buhrke, T ;

Friedrich, B ;

Lubitz, W .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (43) :13075-13083

[4] DFT investigation of structural, electronic, and catalytic properties of diiron complexes related to the [2Fe]H subcluster of Fe-only hydrogenases [J].

Bruschi, M ;

Fantucci, P ;

De Gioia, L .

INORGANIC CHEMISTRY, 2002, 41 (06) :1421-1429

[5] Density functional theory investigation of the active site of [Fe]-hydrogenases:: Effects of redox state and ligand characteristics on structural, electronic, and reactivity properties of complexes related to the [2Fe]H subcluster [J].

Bruschi, M ;

Fantucci, P ;

De Gioia, L .

INORGANIC CHEMISTRY, 2003, 42 (15) :4773-4781

[6]

Cammack R., 2001, Hydrogen as a Fuel Learning from Nature

[7] 17O ENDOR detection of a solvent-derived Ni-(OHx)-Fe bridge that is lost upon activation of the hydrogenase from Desulfovibrio gigas [J].

Carepo, M ;

Tierney, DL ;

Brondino, CD ;

Yang, TC ;

Pamplona, A ;

Telser, J ;

Moura, I ;

Moura, JJG ;

Hoffman, BM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (02) :281-286

[8] Infrared studies of the CO-inhibited form of the Fe-only hydrogenase from Clostridium pasteurianum I:: Examination of its light sensitivity at cryogenic temperatures [J].

Chen, ZJ ;

Lemon, BJ ;

Huang, S ;

Swartz, DJ ;

Peters, JW ;

Bagley, KA .

BIOCHEMISTRY, 2002, 41 (06) :2036-2043

[9] The organometallic active site of [Fe]hydrogenase: Models and entatic states [J].

Darensbourg, MY ;

Lyon, EJ ;

Zhao, X ;

Georgakaki, IP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3683-3688

[10] Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovorans Ni-Fe hydrogenase [J].

DeLacey, AL ;

Fernandez, VM ;

Rousset, M ;

Cavazza, C ;

Hatchikian, EC .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2003, 8 (1-2) :129-134

← 1 2 3 4 5 →