ESTABLISHMENT OF A COMPETITIVE ELISA FOR DETECTION OF FLORFENICOL ANTIBIOTIC IN FOOD OF ANIMAL ORIGIN

被引:13
作者
Sheu, Shi-Yuan [1 ,2 ,3 ]
Wang, Yueh-Kuei [4 ]
Tai, Yung-Te [5 ]
Lei, Yi-Chih [4 ]
Chang, Tong-Hsuan [5 ]
Yao, Chun-Hsu [6 ]
Kuo, Tzong-Fu [4 ]
机构
[1] China Med Univ, Sch Chinese Med, Taichung, Taiwan
[2] Chung Shan Med Univ, Sch Med, Taichung, Taiwan
[3] Chung Shan Med Univ Hosp, Dept Integrated Chinese & Western Med, Taichung, Taiwan
[4] Natl Taiwan Univ, Sch Vet Med, Dept & Grad Inst Vet, Taipei 10764, Taiwan
[5] Taiwan Adv Biopharmaceut Inc, New Taipei City, Taiwan
[6] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung, Taiwan
关键词
enzyme-linked immunosorbent assay; florfenicol; food safety; hapten; residues; LINKED-IMMUNOSORBENT-ASSAY; GAS-CHROMATOGRAPHY; AMINE; CHLORAMPHENICOL; THIAMPHENICOL; RESIDUES; METABOLITE; MUSCLE; FISH; EFFICACY;
D O I
10.1080/15321819.2013.779924
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Florfenicol (FF) is a synthetic antibiotic with a broad antibacterial spectrum and the high therapeutic effectiveness that has been developed specifically for veterinary use. Obviously, FF adulterated in animal supplies is one of essential global concerns. A competitive ELISA for the detection of florfenicol in food of animal origin (swine, chicken, and fish) is described. Influence of immunoconjugate structure on the assay sensitivity and specificity was investigated. The new ELISA showed much lower than the MRPLs for FF at 100-3,000mgkg(-1) in the European Communities and the sensitivity of our ELISA method was superior to that described in other reports. According to the test preparation record, the limit of detection of the developed ELISA performed on meat species was 0.3 mu gkg(-1) (IC50 value 1.9 mu gkg(-1)). The method developed permits FF concentrations to be determined in the range 0.3-24.3 mu gkg(-1). A low cross-reactivity with florfenicol amine (FFA), thiamphenicol (TAP), and chloramphenicol (CAP) was displayed (16.2%, 9.5%, and 9.4%, respectively). Recovery in different food samples (swine, chicken, and fish) averages between 87-115%. The method can be applied for inspection of animal supplies for trace florfenicol residues.
引用
收藏
页码:438 / 452
页数:15
相关论文
共 33 条
[1]   Simultaneous Determination of 20 Pharmacologically Active Substances in Cow's Milk, Goat's Milk, and Human Breast Milk by Gas Chromatography-Mass Spectrometry [J].
Azzouz, Abdelmonaim ;
Jurado-Sanchez, Beatriz ;
Souhail, Badredine ;
Ballesteros, Evaristo .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (09) :5125-5132
[2]   Determination of residual pharmaceuticals in edible animal tissues by continuous solid-phase extraction and gas chromatography-mass spectrometry [J].
Azzouz, Abdelmonaim ;
Souhail, Badredine ;
Ballesteros, Evaristo .
TALANTA, 2011, 84 (03) :820-828
[3]   Development and Residue Screening of the Furazolidone Metabolite, 3-Amino-2-oxazolidinone (AOZ), in Cultured Fish by an Enzyme-Linked Immunosorbent Assay [J].
Cheng, Chu-Chen ;
Hsieh, Kuan-Huei ;
Lei, Yi-Chih ;
Tai, Yung-Te ;
Chang, Tong-Hsuan ;
Sheu, Shi-Yuan ;
Li, Wen-Ren ;
Kuo, Tzong-Fu .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (13) :5687-5692
[4]  
EMEA Florfenicol, 2002, EMEA82202FINAL
[5]   INVITRO AND INVIVO ANTIBACTERIAL ACTIVITIES OF FLORFENICOL, A NEW FLUORINATED ANALOG OF THIAMPHENICOL, AGAINST FISH PATHOGENS [J].
FUKUI, H ;
FUJIHARA, Y ;
KANO, T .
FISH PATHOLOGY, 1987, 22 (04) :201-207
[6]   CHLORAMPHENICOL-SPECIFIC ANTIBODY [J].
HAMBURGE.RN .
SCIENCE, 1966, 152 (3719) :203-&
[7]  
Hayes JM, 2005, J AOAC INT, V88, P1777
[8]  
Hoar BR, 1998, CAN VET J, V39, P161
[9]   Simultaneous extraction and determination of florfenicol and the metabolite florfenicol amine in sediment by high-performance liquid chromatography [J].
Hormazabal, V ;
Steffenak, I ;
Yndestad, M .
JOURNAL OF CHROMATOGRAPHY A, 1996, 724 (1-2) :364-366
[10]   SIMULTANEOUS DETERMINATION OF RESIDUES OF FLORFENICOL AND THE METABOLITE FLORFENICOL AMINE IN FISH-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
HORMAZABAL, V ;
STEFFENAK, I ;
YNDESTAD, M .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 616 (01) :161-165