The effects of silencing of PI3K p85α on 5-FU-induced colorectal cancer cells apoptosis

被引：7

作者：

Sun, Yan ^{[1
]}

Tian, Hua ^{[2
]}

Wang, Lin ^{[3
]}

Yang, Hui ^{[4
]}

机构：

[1] Guangzhou Med Univ, Dept Gastroenterol, Affiliated Hosp 3, Guangzhou 510150, Guangdong, Peoples R China

[2] Guangdong Med Coll, Houjie Hosp, Dept Gastroenterol, Dongguan 523900, Peoples R China

[3] Guang Zhou Red Cross Hosp, Dept Oncol, Guangzhou 510150, Guangdong, Peoples R China

[4] Guangdong 999 Brain Hosp, Dept Neurol, Guangzhou 510510, Guangdong, Peoples R China

来源：

MEDICAL ONCOLOGY | 2013年 / 30卷 / 04期

关键词：

PI3K p85 alpha; Colorectal cancer; Hoechst; 33258; staining; JC-1 mitochondrial membrane potential; FLUOROURACIL PLUS LEUCOVORIN; PHOSPHATIDYLINOSITOL 3'-KINASE; PATHWAY; INHIBITION; SUBUNIT; OVARIAN; GENE;

D O I：

10.1007/s12032-013-0704-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Colorectal cancer is the third most common malignancy worldwide. 5-fluorouracil (5-FU) is the commonly used chemotherapeutic agent, however, more patients develop resistance. Phosphatidylinositol 3-kinases (PI3Ks) play a crucial role in a wide range of cellular processes associated with malignant behavior including cell growth, migration, and survival. In this study, we show increased expression of PI3K p85 alpha during the progression of colorectal cancer. Silencing of PI3K p85 alpha in colorectal cancer cells increased disruption of mitochondrial membrane potential and enhanced 5-FU-induced apoptosis. Furthermore, PI3K p85 alpha-depletion results in activated expression of apoptosis-associated genes Bcl-6, Bim, and Bax. Our results suggest that knockdown of PI3K p85 alpha is a potential therapeutic strategy in the treatment of colorectal cancer.

引用

页数：8

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