Quantitative evaluation of thin-layer chromatography with image background estimation based on charge-coupled device imaging

被引：29
作者：
Zhang, L [1 ]
Lin, XG [1 ]
机构：
[1] Tsing Hua Univ, Dept Elect Engn, Beijing 100084, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1109卷 / 02期
关键词：
quantitative evaluation; TLC; CCD imaging; cubic spline surface; image background estimation;
D O I：
10.1016/j.chroma.2006.01.018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The main limitation of image processing based on charge-coupled device (CCD) imaging in quantitative evaluation of thin-layer chromatography (TLC) is its nonuniform illumination. This paper presents a novel method to solve the problem. First, the background is estimated by employing the cubic spline surface construction. Then the estimated background is subtracted from the original image to make the image segmentation; Finally, the gray level integration for each object is calculated, which is proportional to the value of samples. Experiment shows that the linear correlation coefficient between gray integration and concentration of samples is 0.9978. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：273 / 278
页数：6
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