Partial heat denaturation step during reverse transcription and PCR screening yields full-length 5′-cDNAs

被引:18
作者
Hüttemann, M [1 ]
机构
[1] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI 48201 USA
关键词
D O I
10.2144/02324bm02
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
[No abstract available]
引用
收藏
页码:730 / +
页数:4
相关论文
共 6 条
[1]  
Draper DE, 1995, METHOD ENZYMOL, V259, P281
[2]  
Frohman Michael A., 1995, P381
[3]   The cDNA sequences of cytochrome c oxidase subunit VIa from carp and rainbow trout suggest the absence of isoforms in fishes [J].
Huttemann, M ;
Exner, S ;
Arnold, S ;
Lottspeich, F ;
Kadenbach, B .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1997, 1319 (01) :14-18
[4]   New isoforms of cytochrome c oxidase subunit IV in tuna fish [J].
Hüttemann, M .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2000, 1492 (01) :242-246
[5]   RNA structure and the regulation of gene expression [J].
Klaff, P ;
Riesner, D ;
Steger, G .
PLANT MOLECULAR BIOLOGY, 1996, 32 (1-2) :89-106
[6]   FUSION POINT OF HYBRID DNA-RNA MOLECULES [J].
WEGNEZ, M ;
DENIS, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 204 (02) :617-+