HPLC METHOD FOR SCREENING OF STEROIDAL SAPONINS AND RUTIN AS BIOLOGICALLY ACTIVE COMPOUNDS IN TRIBULUS TERRESTRIS L.

被引:11
作者
Ivanova, A. [1 ]
Lazarova, I. [1 ]
Mechkarova, P. [1 ]
Tchorbanov, B. [1 ]
机构
[1] Bulgarian Acad Sci, Ctr Phytochem, Inst Organ Chem, BU-1113 Sofia, Bulgaria
关键词
Tribulus terrestris; ultrasound extraction; steroidal saponin; HPLC;
D O I
10.1080/13102818.2010.10817826
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An improvement method for extraction of steroidal saponins and rutin of Tribulus terrestris L. (Zygophyllaceae) was evaluated. It is shown that the ultrasound extraction is faster, easier, solvent-saving, more reliable and effective method than the conventional heat reflux extraction method. Under optimized conditions, the main biologically active compounds protodioscin, prototribestin, dioscin and rutin were extracted from Tribulus terrestris with different origin and analyzed by revesed phase HPLC with UV detector.
引用
收藏
页码:129 / 133
页数:5
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