Silencing TGIF suppresses migration, invasion and metastasis of MDA-MB-231 human breast cancer cells

被引：14
作者：
Wang, Yadong [1 ,2 ]
Li, Li [1 ]
Wang, Haiyu [1 ]
Li, Jiangmin [1 ]
Yang, Haiyan [3 ]
机构：
[1] Henan Ctr Dis Control & Prevent, Dept Toxicol, 105 South Nongye Rd, Zhengzhou 450016, Henan, Peoples R China
[2] Xinxiang Med Univ, Henan Collaborat Innovat Ctr Mol Diag & Lab Med, Xinxiang 453003, Henan, Peoples R China
[3] Zhengzhou Univ, Sch Publ Hlth, Dept Epidemiol, 100 Sci Rd, Zhengzhou 450001, Henan, Peoples R China
来源：
ONCOLOGY REPORTS | 2018年 / 39卷 / 02期
基金：
中国国家自然科学基金;
关键词：
TGIF; migration; invasion; metastasis; MDA-MB-231; breast cancer; INTERACTING FACTOR; SUPEROXIDE-PRODUCTION; DUCTAL CARCINOMA; DOWN-REGULATION; BETA-CATENIN; EXPRESSION; SNAIL1; GROWTH; MMP-2; OVEREXPRESSION;
D O I：
10.3892/or.2017.6133
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
This study explored the potential role of TG-interacting factor (TGIF) in migration, invasion and metastasis of the human breast cancer cell line MDA-MB-231. Western blot assay, immunohistochemistry and qRT-PCR assays were applied to detect the expression of protein and mRNA. Wound healing assay, Transwell invasion assay and tail vein metastatic assay were performed to assess the migration, invasion and metastasis of stable TGIF-silenced MDA-MB-231 cell line in vitro and in vivo. The significantly higher frequency of TGIF high-expression was observed in metastatic breast cancer (62.9%) compared to that in non-metastatic breast cancer (25.8%). Silencing TGIF suppressed migration and invasion of MDA-MB-231 cells in vitro and tumor metastasis in nude mouse models. The expression of Snail1, matrix metalloproteinase 2 (MMP2) and beta-catenin was markedly decreased in the stable TGIF-silenced MDA-MB-231 cells compared with the control cells. Our results suggest that silencing TGIF suppressed the migration, invasion and metastasis of the human breast cancer cell line MDA-MB-231 using in vitro and in vivo experiments.
引用
收藏
页码：802 / 808
页数：7
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