Arrhythmic Gut Microbiome Signatures Predict Risk of Type 2 Diabetes

被引：195

作者：

Reitmeier, Sandra ^{[1
,2
]}

Kiessling, Silke ^{[1
,2
]}

Clavel, Thomas ^{[1
,3
]}

List, Markus ^{[4
]}

Almeida, Eduardo L. ^{[5
]}

Ghosh, Tarini S. ^{[5
]}

Neuhaus, Klaus ^{[1
]}

Grallert, Harald ^{[6
,7
]}

Linseisen, Jakob ^{[6
,7
,8
]}

Skurk, Thomas ^{[1
]}

Brandl, Beate ^{[1
]}

Breuninger, Taylor A. ^{[6
,7
]}

Troll, Martina ^{[6
,7
]}

Rathmann, Wolfgang ^{[10
,11
]}

Linkohr, Birgit ^{[6
,7
]}

Hauner, Hans ^{[1
,9
]}

Laudes, Matthias ^{[12
,13
]}

Franke, Andre ^{[12
]}

Le Roy, Caroline, I ^{[14
]}

Bell, Jordana T. ^{[14
]}

Spector, Tim ^{[14
]}

Baumbach, Jan ^{[4
]}

O'Toole, Paul W. ^{[5
]}

Peters, Annette ^{[6
,7
,15
]}

Haller, Dirk ^{[1
,2
]}

机构：

[1] Tech Univ Munich, ZIEL Inst Food & Hlth, D-85354 Freising Weihenstephan, Germany

[2] Tech Univ Munich, Chair Nutr & Immunol, Gregor Mendel Str 2, D-85354 Freising Weihenstephan, Germany

[3] RWTH Univ Hosp, Inst Med Microbiol, Funct Microbiome Res Grp, Pauwelsstr 30, D-52074 Aachen, Germany

[4] Tech Univ Munich, Chair Expt Bioinformat, Maximus von Imhof Forum 3, D-85354 Freising Weihenstephan, Germany

[5] Univ Coll Cork, Sch Microbiol & APC Microbiome Ireland, Cork, Ireland

[6] Helmholtz Zentrum Munchen, Ingolstaedter Landstr 1, D-85764 Oberschleissheim, Germany

[7] German Diabet Ctr DDZ, Ingolstaedter Landstr 1, D-85764 Oberschleissheim, Germany

[8] Ludwig Maximilians Univ Munchen, UNIKA T Augsburg, Chair Epidemiol, Neusasser Str 47, D-86156 Augsburg, Germany

[9] Tech Univ Munich, Inst Nutr Med, Georg Brauchle Ring 62, D-80992 Munich, Germany

[10] German Diabet Ctr DDZ, Aufm Hennekamp 65, D-40225 Dusseldorf, Germany

[11] German Ctr Diabet Res DZD eV, Aufm Hennekamp 65, D-40225 Dusseldorf, Germany

[12] Christian Albrechts Univ Kiel, Inst Clin Mol Biol, Rosalind Franklin Str 12, D-24105 Kiel, Germany

[13] Univ Kiel, Dept Med, Div Endocrinol Diabet & Clin Nutr, D-24105 Kiel, Germany

[14] Kings Coll London, Dept Twin Res & Genet Epidemiol, London WC2R 2LS, England

[15] Ludwig Maximilians Univ Munchen, Chair Epidemiol, Marchioninistr 15, D-81377 Munich, Germany

来源：

CELL HOST & MICROBE | 2020年 / 28卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金; 爱尔兰科学基金会;

关键词：

INTESTINAL MICROBIOTA; CIRCADIAN CLOCK; RHYTHMS; METAGENOME; SEQUENCES; MELLITUS; DATABASE;

D O I：

10.1016/j.chom.2020.06.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lifestyle, obesity, and the gut microbiome are important risk factors for metabolic disorders. We demonstrate in 1,976 subjects of a German population cohort (KORA) that specific microbiota members show 24-h oscillations in their relative abundance and identified 13 taxa with disrupted rhythmicity in type 2 diabetes (T2D). Cross-validated prediction models based on this signature similarly classified T2D. In an independent cohort (FoCus), disruption of microbial oscillation and the model for T2D classification was confirmed in 1,363 subjects. This arrhythmic risk signature was able to predict T2D in 699 KORA subjects 5 years after initial sampling, being most effective in combination with BMI, Shotgun metagenomic analysis functionally linked 26 metabolic pathways to the diurnal oscillation of gut bacteria. Thus, a cohort-specific risk pattern of arrhythmic taxa enables classification and prediction of T2D, suggesting a functional link between circadian rhythms and the microbiome in metabolic diseases.

引用

页码：258 / +

页数：21

共 66 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] Enterotypes of the human gut microbiome [J].

Arumugam, Manimozhiyan ;

Raes, Jeroen ;

Pelletier, Eric ;

Le Paslier, Denis ;

Yamada, Takuji ;

Mende, Daniel R. ;

Fernandes, Gabriel R. ;

Tap, Julien ;

Bruls, Thomas ;

Batto, Jean-Michel ;

Bertalan, Marcelo ;

Borruel, Natalia ;

Casellas, Francesc ;

Fernandez, Leyden ;

Gautier, Laurent ;

Hansen, Torben ;

Hattori, Masahira ;

Hayashi, Tetsuya ;

Kleerebezem, Michiel ;

Kurokawa, Ken ;

Leclerc, Marion ;

Levenez, Florence ;

Manichanh, Chaysavanh ;

Nielsen, H. Bjorn ;

Nielsen, Trine ;

Pons, Nicolas ;

Poulain, Julie ;

Qin, Junjie ;

Sicheritz-Ponten, Thomas ;

Tims, Sebastian ;

Torrents, David ;

Ugarte, Edgardo ;

Zoetendal, Erwin G. ;

Wang, Jun ;

Guarner, Francisco ;

Pedersen, Oluf ;

de Vos, Willem M. ;

Brunak, Soren ;

Dore, Joel ;

Weissenbach, Jean ;

Ehrlich, S. Dusko ;

Bork, Peer .

NATURE, 2011, 473 (7346) :174-180

[3] Heatmapper: web-enabled heat mapping for all [J].

Babicki, Sasha ;

Arndt, David ;

Marcu, Ana ;

Liang, Yongjie ;

Grant, Jason R. ;

Maciejewski, Adam ;

Wishart, David S. .

NUCLEIC ACIDS RESEARCH, 2016, 44 (W1) :W147-W153

[4] Metformin affects the circadian clock and metabolic rhythms in a tissue-specific manner [J].

Barnea, Maayan ;

Haviv, Liyan ;

Gutman, Roee ;

Chapnik, Nava ;

Madar, Zecharia ;

Froy, Oren .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2012, 1822 (11) :1796-1806

[5] Loss of Diurnal Oscillatory Rhythms in Gut Microbiota Correlates with Changes in Circulating Metabolites in Type 2 Diabetic db/db Mice [J].

Beli, Eleni ;

Prabakaran, Samantha ;

Krishnan, Preethi ;

Evans-Molina, Carmella ;

Grant, Maria B. .

NUTRIENTS, 2019, 11 (10)

[6] Barcoded Primers Used in Multiplex Amplicon Pyrosequencing Bias Amplification [J].

Berry, David ;

Ben Mahfoudh, Karim ;

Wagner, Michael ;

Loy, Alexander .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (21) :7846-7849

[7]

Biecek P., 2018, J. Mach.Learn. Res, V19, P1, DOI [DOI 10.1021/ACS.MOLPHARMACEUT.7B00346, DOI 10.48550/ARXIV.1806.08915]

[8] Random forests [J].

Breiman, L .

MACHINE LEARNING, 2001, 45 (01) :5-32

[9] Timing of food intake impacts daily rhythms of human salivary microbiota: a randomized, crossover study [J].

Carmen Collado, Maria ;

Engen, Phillip A. ;

Bandin, Cristina ;

Cabrera-Rubio, Raul ;

Voigt, Robin M. ;

Green, Stefan J. ;

Naqib, Ankur ;

Keshavarzian, Ali ;

Scheer, Frank A. J. L. ;

Garaulet, Marta .

FASEB JOURNAL, 2018, 32 (04) :2060-2072

[10]

Edgar RC, 2013, NAT METHODS, V10, P996, DOI [10.1038/NMETH.2604, 10.1038/nmeth.2604]

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