Identification and Characterization of Two Novel Staphylococcal Enterotoxins, Types S and T

被引:160
作者
Ono, Hisaya K. [1 ]
Omoe, Katsuhiko [1 ]
Imanishi, Ken'ichi [2 ]
Iwakabe, Yoshihiro [1 ]
Hu, Dong-Liang [3 ]
Kato, Hidehito [2 ]
Saito, Naoyuki [4 ]
Nakane, Akio [3 ]
Uchiyama, Takehiko [2 ]
Shinagawa, Kunihiro [1 ]
机构
[1] Iwate Univ, Fac Agr, Dept Vet Med, Morioka, Iwate 0208550, Japan
[2] Tokyo Womens Med Univ, Sch Med, Dept Microbiol & Immunol, Shinjuku Ku, Tokyo 1628666, Japan
[3] Hirosaki Univ, Grad Sch Med, Dept Microbiol & Immunol, Hirosaki, Aomori 0368562, Japan
[4] Corp Prod & Res Lab Primates, Tsukuba, Ibaraki 3050843, Japan
基金
日本学术振兴会;
关键词
D O I
10.1128/IAI.00045-08
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In addition to two known staphylococcal enterotoxin-like genes (selj and selr), two novel genes coding for two superantigens, staphylococcal enterotoxins S and T (SES and SET), were identified in plasmid pF5, which is harbored by food poisoning-related Staphylococcus aureus strain Fukuoka 5. This strain was implicated in a food poisoning incident in Fukuoka City, Japan, in 1997. Recombinant SES (rSES) specifically stimulated human T cells in a T-cell receptor V beta 9- and V beta 16-specific manner in the presence of major histocompatibility complex (MHC) class II+ antigen-presenting cells (APC). rSET also stimulated T cells in the presence of MHC class II+ APC, although its V beta skewing was not found in reactive T cells. Subsequently, we examined the emetic activity of SES and SET. We also studied SElR to determine emetic activity in primates. This toxin was identified in previous studies but was not examined in terms of possession of emetic activity for primates. rSES induced emetic reactions in two of four monkeys at a dose of 100 mu g/kg within 5 h of intragastric administration. In one monkey, rSET induced a delayed reaction (24 h postadministration) at a dose of 100 mu g/kg, and in the other one, the reaction occurred 5 days postadministration. rSElR induced a reaction in two of six animals within 5 h at 100 mu g/kg. On this basis, we speculate that the causative toxins of vomiting in the Fukuoka case are SES and SER. Additionally, SES, SER, and SET also induced emesis in house musk shrews as in the monkeys.
引用
收藏
页码:4999 / 5005
页数:7
相关论文
共 33 条
[1]   The three-dimensional structure of a superantigen-like protein, SET3, from a pathogenicity island of the Staphylococcus aureus genome [J].
Arcus, VL ;
Langley, R ;
Proft, T ;
Fraser, JD ;
Baker, EN .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (35) :32274-32281
[2]   Genome and virulence determinants of high virulence community-acquired MRSA [J].
Baba, T ;
Takeuchi, F ;
Kuroda, M ;
Yuzawa, H ;
Aoki, K ;
Oguchi, A ;
Nagai, Y ;
Iwama, N ;
Asano, K ;
Naimi, T ;
Kuroda, H ;
Cui, L ;
Yamamoto, K ;
Hiramatsu, K .
LANCET, 2002, 359 (9320) :1819-1827
[3]  
Bergdoll M. S., 1989, Foodborne bacterial pathogens., P463
[4]  
BERGDOLL MS, 1988, METHOD ENZYMOL, V165, P324
[5]   Enterotoxins and toxic shock syndrome toxin in Staphylococcus aureus recovered from human nasal carriers and manually handled foods:: epidemiological and genetic findings [J].
Fueyo, JM ;
Mendoza, MC ;
Martín, MC .
MICROBES AND INFECTION, 2005, 7 (02) :187-194
[6]   Induction of emetic response to staphylococcal enterotoxins in the house musk shrew (Suncus murinus) [J].
Hu, DL ;
Omoe, K ;
Shimoda, Y ;
Nakane, A ;
Shinagawa, K .
INFECTION AND IMMUNITY, 2003, 71 (01) :567-570
[7]   Staphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is downregulated by cannabinoid receptor 1 [J].
Hu, Dong-Liang ;
Zhu, Gang ;
Mori, Fumiaki ;
Omoe, Katsuhiko ;
Okada, Motohiro ;
Wakabayashi, Koichi ;
Kaneko, Sunao ;
Shinagawa, Kunihiro ;
Nakane, Akio .
CELLULAR MICROBIOLOGY, 2007, 9 (09) :2267-2277
[8]   Maturation of adult peripheral blood CD38+CD4+ T cells demonstrated by cytokine production in response to a superantigen, TSST-1 [J].
Imanishi, K ;
Kato, H ;
Fujii, H ;
Uchiyama, T .
CELLULAR IMMUNOLOGY, 2003, 222 (02) :89-96
[9]  
Imanishi K, 1998, J IMMUNOL, V160, P112
[10]   egc, A highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus [J].
Jarraud, S ;
Peyrat, MA ;
Lim, A ;
Tristan, A ;
Bes, M ;
Mougel, C ;
Etienne, J ;
Vandenesch, F ;
Bonneville, M ;
Lina, G .
JOURNAL OF IMMUNOLOGY, 2001, 166 (01) :669-677