Parallel acquisition of multi-dimensional spectra in protein NMR

We introduce the use of multiple receivers applied in parallel for simultaneously recording multi-dimensional data sets of proteins in a single experiment. The utility of the approach is established through the introduction of the 2D N-15,H-1(N)||(CO)-C-13 HSQC experiment in which a pair of two-dimensional N-15,H-1(N) and N-15,(CO)-C-13 spectra are recorded. The methodology is further extended to higher dimensionality via the 3D H-1(N)||(CO)-C-13 HNCA in which a pair of data sets recording C-13(alpha),N-15,H-1(N) and C-13(alpha),N-15,(CO)-C-13 chemical shifts are acquired. With the anticipated increases in probe sensitivity it is expected that multiple receiver experiments will become an important approach for efficient recording of NMR data.