DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers

被引：16

作者：

Shtenberg, Giorgi ^{[3
]}

Massad-Ivanir, Naama ^{[1
]}

Engin, Sinem ^{[4
]}

Sharon, Michal ^{[5
]}

Fruk, Ljiljana ^{[4
]}

Segal, Ester ^{[1
,2
]}

机构：

[1] Technion Israel Inst Technol, Dept Biotechnol & Food Engn, IL-32000 Haifa, Israel

[2] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-32000 Haifa, Israel

[3] Technion Israel Inst Technol, Interdept Program Biotechnol, IL-32000 Haifa, Israel

[4] Karlsruhe Inst Technol, DFG Ctr Funct Nanostruct, D-76131 Karlsruhe, Germany

[5] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

来源：

NANOSCALE RESEARCH LETTERS | 2012年 / 7卷

关键词：

Porous Si; DNA immobilization; Enzyme; Nanostructure; Biosensor; SILICON DOUBLE-LAYER; ENZYME-ACTIVITY; BACTERIA;

D O I：

10.1186/1556-276X-7-443

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-P,rot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild dehybridization conditions.

引用

页数：6

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