Label-free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme

被引：29

作者：

Wang, Haiyan ^{[1
]}

Gong, Wu ^{[1
]}

Tan, Zhian ^{[1
]}

Yin, Xunxun ^{[1
]}

Wang, Lun ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Anhui Key Lab Chemobiosensing, Wuhu 241000, Peoples R China

来源：

ELECTROCHIMICA ACTA | 2012年 / 76卷

基金：

中国国家自然科学基金;

关键词：

Electrochemiluminescence; Aptasensor; Ru(phen)(3)(2+); Adenosine; Lysozyme; ELECTROCHEMICAL BIOSENSOR; GOLD NANOPARTICLES; APTAMER; DNA; AMPLIFICATION; ELECTRODES; SENSORS; ASSAY;

D O I：

10.1016/j.electacta.2012.05.056

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A label-free bifunctional electrochemiluminescence (ECL) aptasensor for parallel detection of small molecule (adenosine) and protein (lysozyme) was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. Thiolated single-stranded DNA (DNA1) containing adenosine aptamer and sequence complementary to DNA2 was first immobilized on the Au electrode via sulfur-gold affinity. DNA2 containing lysozyme aptamer and sequence complememtary to DNA3 was bound to the electrode via forming DNA/DNA duplex. Gold nanoparticles functionalized with DNA3 (DNA3-GNP5) were used as signal amplifier to enhance the sensitivity of the aptasensor. With Ru(phen)(3)(2+) as the signal transducer, the aptasensor exhibited high sensitivity and specificity. Under optimal conditions, a linear range from 0.5 to 7 nM for adenosine (R=0.999) and from 0.5 to 9 ng/mL for lysozyme (R = 0.999) was obtained, respectively. The proposed method may provide a promising platform for ECL parallel detection of small molecules and protein. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：416 / 423

页数：8

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