Differential Toxicity of Rod and Spherical Zinc Oxide Nanoparticles on Human Peripheral Blood Mononuclear Cells

被引：48

作者：

Bhattacharya, Debalina ^{[1
]}

Santra, Chitta Ranjan ^{[2
]}

Ghosh, Amar N. ^{[3
]}

Karmakar, Parimal ^{[1
]}

机构：

[1] Jadavpur Univ, Dept Life Sci & Biotechnol, Kolkata 700032, W Bengal, India

[2] Netaji Nagar Day Coll, Dept Chem, Kolkata 700092, W Bengal, India

[3] Natl Inst Cholera & Enter Dis, Kolkata 700010, W Bengal, India

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2014年 / 10卷 / 04期

关键词：

Zinc Oxide Nanoparticles; Proliferative Lymphocytes; Reactive Oxygen Species; DNA Damage; Environment; OXIDATIVE STRESS; ZNO PARTICLES; CYTOTOXICITY; APOPTOSIS; SHAPE; TIO2; SIZE;

D O I：

10.1166/jbn.2014.1744

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The cytotoxic and genotoxic potential of rod and spherical zinc oxide nanoparticles (ZnO NPs) were evaluated on human peripheral blood mononuclear cells (PBMCs). Their sizes and shapes were determined by Transmission electron microscopy (TEM) and physical characterizations were done by X-ray diffraction (XRD) and Dynamic light scattering (DLS). The cytotoxic potential of rod ZnO NPs was greater than spherical ZnO NPs when applied to PBMCs and was limited to the proliferative lymphocytes. Rod ZnO NPs produced more reactive oxygen species (ROS) compared to spherical ones. Additionally rod ZnO NPs induced significant DNA damage to PBMCs as revealed by cytokinesis block micronucleus (CBMN) assay and comet assay. On the other hand UV absorption property was enhanced in case of spherical ZnO NPs compared to rod ZnO NPs. Thus, present study implicates the shape dependent differential application of ZnO NPs in our daily life.

引用

页码：707 / 716

页数：10

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