Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope

被引：20

作者：

Mills, Bethany ^{[1
]}

Megia-Fernandez, Alicia ^{[2
]}

Norberg, Dominic ^{[1
]}

Duncan, Sheelagh ^{[1
]}

Marshall, Adam ^{[1
]}

Akram, Ahsan R. ^{[1
]}

Quinn, Thomas ^{[1
]}

Young, Irene ^{[1
]}

Bruce, Annya M. ^{[1
]}

Scholefield, Emma ^{[1
]}

Williams, Gareth O. S. ^{[1
]}

Krstajic, Nikola ^{[1
]}

Choudhary, Tushar R. ^{[1
,3
,4
]}

Parker, Helen E. ^{[1
,5
]}

Tanner, Michael G. ^{[1
,6
]}

Harrington, Kerrianne ^{[7
]}

Wood, Harry A. C. ^{[7
]}

Birks, Timothy A. ^{[7
]}

Knight, Jonathan C. ^{[7
]}

Haslett, Christopher ^{[1
]}

Dhaliwal, Kevin ^{[1
]}

Bradley, Mark ^{[2
]}

Ucuncu, Muhammed ^{[2
,8
]}

Stone, James M. ^{[7
]}

机构：

[1] Univ Edinburgh, Queens Med Res Inst, Ctr Inflammat Res, 47 Little France Crescent, Edinburgh EH16 4TJ, Midlothian, Scotland

[2] Univ Edinburgh, Sch Chem, Joseph Black Bldg,David Brewster Rd, Edinburgh EH9 3FJ, Midlothian, Scotland

[3] Univ Edinburgh, Roslin Inst, Edinburgh, Midlothian, Scotland

[4] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh, Midlothian, Scotland

[5] Royal Inst Technol, Dept Appl Phys, KTH, SE-10691 Stockholm, Sweden

[6] Heriot Watt Univ, Scottish Univ Phys Alliance SUPA, Inst Photon & Quantum Sci, Edinburgh EH14 4AS, Midlothian, Scotland

[7] Univ Bath, Dept Phys, Ctr Photon & Photon Mat, Bath BA2 7AY, Avon, England

[8] Izmir Katip Celebi Univ, Dept Analyt Chem, Fac Pharm, Izmir, Turkey

来源：

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | 2021年 / 48卷 / 03期

基金：

英国工程与自然科学研究理事会; 英国医学研究理事会;

关键词：

Optical imaging; Fluorescence; Bacteria; Gram-positive; Lung; Optical endomicroscopy; VENTILATOR-ASSOCIATED PNEUMONIA; GLYCOPEPTIDE ANTIBIOTICS; MEROCYANINE DYES; MICROBIOME; INFECTIONS; RESISTANCE; DIAGNOSIS;

D O I：

10.1007/s00259-020-05021-4

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Purpose The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, a better understanding of bacterial colonization and infection. To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber-based delivery/imaging endoscopic device. Methods We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system. Results The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging ofS. aureuswithin the alveolar space of whole ex vivo human lungs within 60 s of delivery into the field-of-view, using the novel imaging/delivery endomicroscopy device. Conclusion This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.

引用

页码：800 / 807

页数：8

共 29 条

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