The ββα fold of zinc finger proteins as a "natural" protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative

被引：15

作者：

Rodriguez, Jessica ^{[1
]}

Mosquera, Jesus ^{[1
]}

Vazquez, Olalla ^{[1
]}

Eugenio Vazquez, M. ^{[1
]}

Mascarenas, Jose L. ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Ctr Singular Invest Quim Biol & Mat Mol CIQUS, Dept Quim Organ, Santiago De Compostela 15782, Spain

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 18期

关键词：

MINOR-GROOVE; POSTTRANSLATIONAL MODIFICATIONS; CHEMICAL-MODIFICATION; AMINO-ACIDS; PEPTIDE; RECOGNITION; CHEMISTRY; NUCLEASES; SEQUENCES; VERSATILE;

D O I：

10.1039/c3cc47599a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys(2)His(2) zinc finger motif. The chemoselective modification is achieved, thanks to the protecting effect exerted by the zinc cation upon coordination with the native cysteines and histidines of the zinc-finger fold. The strategy allows a straightforward synthesis of DNA binding zinc finger constructs.

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页码：2258 / 2260

页数：3

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