Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress

被引:27
作者
Cevik, O. [1 ]
Cadirci, S. [2 ]
Sener, T. E. [3 ]
Tinay, I. [3 ]
Akbal, C. [3 ]
Tavukcu, H. H. [4 ]
Cetinel, S. [5 ]
Kiran, D. [5 ]
Sener, G. [2 ]
机构
[1] Cumhuriyet Univ, Sch Pharm, Dept Biochem, Sivas, Turkey
[2] Marmara Univ, Sch Pharm, Dept Pharmacol, TR-34668 Istanbul, Turkey
[3] Marmara Univ, Sch Med, Dept Urol, TR-34668 Istanbul, Turkey
[4] Acad Hosp, Dept Urol, Istanbul, Turkey
[5] Marmara Univ, Sch Med, Dept Histol & Embryol, TR-34668 Istanbul, Turkey
关键词
quercetin; ischemia/reperfusion; antioxidant; apoptosis; inflammation; corpus cavernosum; ERECTILE DYSFUNCTION; PENILE ARTERIES; PROTECTIVE ROLE; NITRIC-OXIDE; ISCHEMIA; ANTIOXIDANT; MELATONIN; MYELOPEROXIDASE; INFLAMMATION; MECHANISMS;
D O I
10.3109/10715762.2013.814912
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reactive oxygen metabolites play an important role in the ischemia/reperfusion (I/R)-induced tissue injury. This study was designed to investigate the possible protective effects of quercetin against I/R injury of the rat corpus cavernosum tissue. To induce I/R injury, abdominal aorta was clamped for 30 min and reperfused for 60 min. Quercetin (20 mg/kg) or vehicle was given before ischemia and just after reperfusion in the I/R group and in the sham-operated control group in which clamping was not performed. After decapitation, corpus cavernosum tissues were removed and either placed in organ baths or stored for evaluating biochemical parameters. Oxidative injury was examined by measuring lucigenin chemiluminescence (CL), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities and caspase-3 protein levels. In the I/R group, contractile responses to phenylephrine and relaxation responses to carbachol were impaired significantly compared with those in the control groups, while quercetin treatment in I/R group reversed both of the responses. On the other hand, increase in lucigenin CL, NO, MDA levels and MPO and caspase-3 activities and decrease in GSH levels and SOD activity in the cavernosal tissues of the I/R group were also significantly reversed by quercetin treatment. Furthermore, observed distorted morphology with ruptured endothelial cells and vacuolization in the cytoplasm of cavernosal tissues of I/R no longer persisted in the quercetin-treated I/R group. Thus, our results suggested that treatment with quercetin may have some benefits in controlling I/R-induced tissue injury through its anti-inflammatory, anti-apoptotic, and antioxidant effects.
引用
收藏
页码:683 / 691
页数:9
相关论文
共 41 条
[31]   ERYTHROCYTE SUPEROXIDE-DISMUTASE ACTIVITY AND OTHER PARAMETERS OF COPPER STATUS IN RATS INGESTING LEAD ACETATE [J].
MYLROIE, AA ;
COLLINS, H ;
UMBLES, C ;
KYLE, J .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1986, 82 (03) :512-520
[32]   HYPERCHOLESTEROLEMIA INCREASES ENDOTHELIAL SUPEROXIDE ANION PRODUCTION [J].
OHARA, Y ;
PETERSON, TE ;
HARRISON, DG .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (06) :2546-2551
[33]   Hypoxic relaxation of penile arteries: involvement of endothelial nitric oxide and modulation by reactive oxygen species [J].
Prieto, Dolores ;
Kaminski, Pawel M. ;
Bagi, Zsolt ;
Ahmad, Mansoor ;
Wolin, Michael S. .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2010, 299 (03) :H915-H924
[34]  
Reiter RJ, 2004, POL J PHARMACOL, V56, P159
[35]   Erectile dysfunction and its association with metabolic syndrome and endothelial function among patients with type 2 diabetes mellitus [J].
Ryan, John G. ;
Gajraj, Jason .
JOURNAL OF DIABETES AND ITS COMPLICATIONS, 2012, 26 (02) :141-147
[36]   Protective Role of Quercetin on PCBs-Induced Oxidative Stress and Apoptosis in Hippocampus of Adult Rats [J].
Selvakumar, Kandaswamy ;
Bavithra, Senthamilselvan ;
Suganthi, Muralidharan ;
Benson, Chellakan Selvanesan ;
Elumalai, Perumal ;
Arunkumar, Ramachandran ;
Krishnamoorthy, Gunasekaran ;
Venkataraman, Prabhu ;
Arunakaran, Jagadeesan .
NEUROCHEMICAL RESEARCH, 2012, 37 (04) :708-721
[37]   The effects of melatonin on ischemia-reperfusion induced changes in rat corpus cavernosum [J].
Sener, G ;
Paskaloglu, K ;
Sehirli, AÖ ;
Dülger, GA ;
Alican, I .
JOURNAL OF UROLOGY, 2002, 167 (06) :2624-2627
[38]   Superoxide Anion Production by NADPH Oxidase Plays a Major Role in Erectile Dysfunction in Middle-Aged Rats: Prevention by Antioxidant Therapy [J].
Silva, Fabio H. ;
Monica, Fabiola Z. ;
Bau, Fernando R. ;
Brugnerotto, Ana F. ;
Priviero, Fernanda B. M. ;
Toque, Haroldo A. ;
Antunes, Edson .
JOURNAL OF SEXUAL MEDICINE, 2013, 10 (04) :960-971
[39]   Antioxidant treatment with quercetin ameliorates erectile dysfunction in streptozotocin-induced diabetic rats [J].
Zhang, Wei ;
Wang, Yong ;
Yang, Zengyue ;
Qiu, Jianxin ;
Ma, Jianjun ;
Zhao, Zhiguang ;
Bao, Tingyi .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2011, 112 (03) :215-218
[40]   MECHANISMS OF REPERFUSION INJURY [J].
ZIMMERMAN, BJ ;
GRANGER, DN .
AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1994, 307 (04) :284-292