Profiling of Childhood Adversity-Associated DNA Methylation Changes in Alcoholic Patients and Healthy Controls

被引:32
作者
Zhang, Huiping [1 ,5 ]
Wang, Fan [1 ,5 ]
Kranzler, Henry R. [6 ,7 ]
Zhao, Hongyu [4 ]
Gelernter, Joel [1 ,2 ,3 ,5 ]
机构
[1] Yale Univ, Sch Med, Dept Psychiat, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06510 USA
[3] Yale Univ, Sch Med, Dept Neurobiol, New Haven, CT USA
[4] Yale Univ, Sch Publ Hlth, Div Biostat, New Haven, CT USA
[5] VA Connecticut Healthcare Syst, West Haven, CT USA
[6] Univ Penn, Dept Psychiat, Perelman Sch Med, Philadelphia, PA 19104 USA
[7] Philadelphia VAMC, MIRECC VISN4, Philadelphia, PA USA
来源
PLOS ONE | 2013年 / 8卷 / 06期
基金
美国国家卫生研究院;
关键词
MESSENGER-RNA EXPRESSION; NICOTINE DEPENDENCE; GENE PROMOTER; RISK; HYPERMETHYLATION; GENOTYPE; RECEPTOR; EVENTS; SCHIZOPHRENIA; EPIGENETICS;
D O I
10.1371/journal.pone.0065648
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The increased vulnerability to alcohol dependence (AD) seen in individuals with childhood adversity (CA) may result in part from CA-induced epigenetic changes. To examine CA-associated DNA methylation changes in AD patients, we examined peripheral blood DNA methylation levels of 384 CpGs in promoter regions of 82 candidate genes in 279 African Americans [AAs; 88 with CA (70.5% with AD) and 191 without CA (38.2% with AD)] and 239 European Americans [EAs; 61 with CA (86.9% with AD) and 178 without CA (46.6% with AD)] using Illumina GoldenGate Methylation Array assays. The effect of CA on methylation of individual CpGs and overall methylation in promoter regions of genes was evaluated using a linear regression analysis (with consideration of sex, age, and ancestry proportion of subjects) and a principal components-based analysis, respectively. In EAs, hypermethylation of 10 CpGs in seven genes (ALDH1A1, CART, CHRNA5, HTR1B, OPRL1, PENK, and RGS19) were cross validated in AD patients and healthy controls who were exposed to CA. P values of two CpGs survived Bonferroni correction when all EA samples were analyzed together to increase statistical power [CHRNA5_cg17108064: P-adjust = 2.54 x 10(-5); HTR1B_cg06031989: P-adjust = 8.98 x 10(-5)]. Moreover, overall methylation levels in the promoter regions of three genes (ALDH1A1, OPRL1 and RGS19) were elevated in both EA case and control subjects who were exposed to CA. However, in AAs, CA-associated DNA methylation changes in AD patients were not validated in healthy controls. Our findings suggest that CA could induce population-specific methylation alterations in the promoter regions of specific genes, thus leading to changes in gene transcription and an increased risk for AD and other disorders.
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页数:9
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