Mold species in dust from the International Space Station identified and quantified by mold-specific quantitative PCR

被引：59

作者：

Vesper, Stephen J. ^{[1
]}

Wong, Wing ^{[3
]}

Kuo, C. Mike ^{[2
]}

Pierson, Duane L. ^{[4
]}

机构：

[1] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA

[2] WYLE Labs Inc, Houston, TX USA

[3] Enterprise Advisory Serv Inc, Houston, TX USA

[4] NASA, Lyndon B Johnson Space Ctr, Houston, TX 77058 USA

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 06期

关键词：

Intenational Space Station; mold-specific quantitative PCR; Aspergillus;

D O I：

10.1016/j.resmic.2008.06.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dust was collected over a period of several weeks in 2007 from HEPA filters in the U.S. Laboratory Module of the International Space Station (ISS). The dust was returned on the Space Shuttle Atlantis, mixed, sieved and the DNA was extracted. Using a DNA-based method called mold-specific quantitative PCR (MSQPCR), 39 molds were measured in the dust. Potential opportunistic pathogens Aspergillus flavus and Aspergillus niger and potential moderate toxin producers Penicillium chrysogenum and Penicillium brevicompactum were noteworthy. No cells of the potential opportunistic pathogens Asperqillus fumigatus, Aspergillus terreus, Fusarium solani or Candida albicans were detected. Published by Elsevier Masson SAS.

引用

页码：432 / 435

页数：4

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