C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+T cells

被引：16
作者：
Nabatov, Alexey A. [1 ]
de Jong, MaFein A. W. P. [1 ]
de Witte, Lot [1 ]
Bulgheresi, Silvia [2 ]
Geijtenbeek, Teunis B. H. [1 ]
机构：
[1] Vrije Univ Amsterdam, Med Ctr, Dept Mol Cellular Biol & Immunol, NL-1081 BT Amsterdam, Netherlands
[2] Univ Vienna, Dept Marine Biol, A-1090 Vienna, Austria
来源：
VIROLOGY | 2008年 / 378卷 / 02期
基金：
奥地利科学基金会;
关键词：
gp120; DC-SIGN; microbicides; HIV-1; transmission;
D O I：
10.1016/j.virol.2008.05.025
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp 120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp 120 is in attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the Structural homologue of DC-SIGN, the nematode C-type lectin Mermaid call be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present Oil HIV-1 gp 120 and thereby inhibits HIV-1 binding to DC-SIGN oil DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission From DC to T cells, We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp 120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：323 / 328
页数：6
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