Synergistic Effects from Combination of Cryptotanshinone and Fosfomycin Against Fosfomycin-Susceptible and Fosfomycin-Resistant Staphylococcus aureus

被引：8
作者：
Ruan, Zijing [1 ]
Cui, Jiaqi [2 ,3 ]
He, Zhenqing [2 ]
Guo, Yuting [2 ]
Jia, Xu [2 ]
Huang, Xinhe [1 ]
机构：
[1] Southwest Jiaotong Univ, Sch Life Sci & Engn, 111,North 1st Sect,2nd Ring Rd, Chengdu 610031, Sichuan, Peoples R China
[2] Chengdu Med Coll, Noncoding RNA & Drug Discovery Key Lab Sichuan Pr, Chengdu, Sichuan, Peoples R China
[3] Chengdu Fifth Peoples Hosp, Dept Pathol, Chengdu, Sichuan, Peoples R China
来源：
INFECTION AND DRUG RESISTANCE | 2020年 / 13卷
基金：
中国国家自然科学基金;
关键词：
traditional Chinese medicine; cryptanshinone; fosfomycin; synergistic effect; Staphylococcus aureus; METHICILLIN-RESISTANT; INFECTIONS; TRENDS;
D O I：
10.2147/IDR.S255296
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Purpose: Fosfomycin is now widely used to treat methicillin-resistant S. aureus due to its unique antibacterial activity. However, fosfomycin-resistant S. aureus has rapidly emerged, it is urgent to find new treatments to eliminate fosfomycin-resistant S. aureus infection. The purpose of this study was to analyze the activity of cryptanshinone, a traditional Chinese medicine monomer, in combination with fosfomycin against fosfomycin-sensitive S. aureus (FSSA) and fosfomycin-resistant S. aureus (FRSA). Methods: The MICs of fosfomycin and/or cryptanshinone were determined by agar dilution assay and checkerboard microdilution assay. Furthermore, synergistic effects from fosfomycin and/or cryptanshinone were analyzed by the time-kill assay in vitro. Results: The combination of fosfomycin and cryptotanshinone had a synergistic effect on most (71.43%) of the FRSA and had a partial (28.57%) synergistic effect on a small part. In addition, time sterilization curve verified synergistic activity between cryptanshinone and fosfomycin against FSSA and FRSA, especially when fosfomycin was added for a second time. Conclusion: These data suggest that cryptanshinone combined with fosfomycin could be a novel treatment for FRSA and provide a new direction for the treatment of bacterial infections in the future.
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收藏
页码：2837 / 2844
页数：8
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