A factorial study was conducted with a sandy, petroleum-contaminated subsoil from Ft. Wainwright, Alaska to evaluate the effect of temperature, moisture content, nutrient levels and diesel range hydrocarbon concentration on microbial activity at a cold climate bioremediation site. In soils with low hydrocarbon concentrations (DRO = 500 mg/kg) soil heating and nutrient addition increased biodegradation rates only slightly, suggesting a substrate limitation. In more highly contaminated soil (DRO = 8,100 mg/kg) soil warming increased microbial activity, indicating low soil temperature is the primary factor limiting biodegradation rates at the Ft. Wainwright site. Maximum microbial activity was observed at about 21 degrees C, with little or no additional increase at 31 degrees C, whereas heating the soil to 41 degrees C reduced biodegradation rates. Addition of nitrogen and phosphorus increased biodegradation when the soils were heated above 1 degrees C. Of these two, soil nitrogen concentration was the more important factor in maximizing biodegradation rates.