Sodium-, potassium-, chloride-, and bicarbonate-related effects on blood pressure and electrolyte homeostasis in deoxycorticosterone acetate-treated rats

被引:64
作者
Ziomber, Agata [1 ,2 ]
Machnik, Agnes [1 ,2 ]
Dahlmann, Anke [1 ,2 ]
Dietsch, Peter [3 ]
Beck, Franz-Xaver [4 ]
Wagner, Hubertus [5 ]
Hilgers, Karl F. [1 ,2 ]
Luft, Friedrich C. [6 ]
Eckardt, Kai-Uwe [1 ,2 ]
Titze, Jens [1 ,2 ]
机构
[1] Univ Erlangen Nurnberg, Nikolaus Fiebiger Ctr Mol Med, Gluckstr 6, D-91054 Erlangen, Germany
[2] Univ Erlangen Nurnberg, Dept Hypertens & Nephrol, D-91054 Erlangen, Germany
[3] Charite Campus Benjamin Franklin, Inst Biochem, Berlin, Germany
[4] Univ Munich, Dept Physiol, D-8000 Munich, Germany
[5] Max Rubner Inst, Dept Safety & Qual Meat, Kulmbach, Germany
[6] HELIOS Klinikum Berlin Brandenburg, Max Delbruck Ctr Mol Med, Fac Med Charite, Expt & Clin Res Ctr, Berlin, Germany
关键词
DOCA-salt; salt-induced hypertension; autoregulation; osmotically inactive sodium;
D O I
10.1152/ajprenal.00531.2007
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Na+ loading without Cl- fails to increase blood pressure in the DOCA model. We compared the changes in the total body (TB) effective Na+, K+, Cl-, and water (TBW) content as well as in intracellular (ICV) or extracellular (ECV) volume in rats receiving DOCA-NaCl, DOCA-NaHCO3, or DOCA-KHCO3. We divided 42 male rats into 5 groups. Group 1 was untreated, group 2 received 1% NaCl, and groups 3, 4, and 5 were treated with DOCA and received 1% NaCl, 1.44% NaHCO3, or 1.7% KHCO3 to drink. We measured mean arterial blood pressure (MAP) directly after 3 wk. Tissue electrolyte and water content was measured by chemical analysis. Compared with control rats, DOCA-NaCl increased MAP while DOCA-NaHCO3 and DOCA-KHCO3 did not. DOCA-NaCl increased TBNa+ 26% but only moderately increased TBW. DOCA-NaHCO3 led to similar TBNa+ excess, while TBW and ICV, but not ECV, were increased more than in DOCA-NaCl rats. DOCA-KHCO3 did not affect TBNa+ or volume. At a given TB(Na+ + K+) and TBW, MAP in DOCA-NaCl rats was higher than in control, DOCA-NaHCO3, and DOCA-KHCO3 rats, indicating that hypertension in DOCA-NaCl rats was not dependent on TB(Na+ + K+) and water mass balance. Skin volume retention was hypertonic compared with serum and paralleled hypertension in DOCA-NaCl rats. These rats had higher TB(Na+ + K+)-to-TBW ratio in accumulated fluid than DOCA-NaHCO3 rats. DOCA-NaCl rats also had increased intracellular Cl- concentrations in skeletal muscle. We conclude that excessive cellular electrolyte redistribution and/or intracellular Na+ or Cl- accumulation may play an important role in the pathogenesis of salt-sensitive hypertension.
引用
收藏
页码:F1752 / F1763
页数:12
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