AFM Investigation on Ox-LDL-Induced Changes in Cell Spreading and Cell-Surface Adhesion Property of Endothelial Cells

被引：7
作者：
Shao, Wenxiang [1 ,2 ]
Jin, Hua [3 ]
Huang, Jie [2 ]
Qiu, Bin [1 ]
Xia, Ruihua [4 ]
Deng, Zeyuan [1 ]
Cai, Jiye [3 ]
Chen, Yong [1 ,2 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Peoples R China
[2] Nanchang Univ, Inst Adv Study, Nanchang, Peoples R China
[3] Jinan Univ, Dept Chem, Guangzhou, Guangdong, Peoples R China
[4] Jinan Univ, Dept Elect Informat Engn, Nanchang, Peoples R China
来源：
SCANNING | 2013年 / 35卷 / 02期
基金：
中国国家自然科学基金;
关键词：
atherosclerosis; oxidized low-density lipoprotein (Ox-LDL); human umbilical vein endothelial cells (HUVECs); atomic force microscopy (AFM); cell spreading; adhesive force; LOW-DENSITY-LIPOPROTEIN; ATOMIC-FORCE MICROSCOPY; OXIDIZED-LDL; ATHEROSCLEROSIS; PATHOGENESIS; EXPRESSION; PLASMA; ANTIOXIDANTS; DYSFUNCTION; MOLECULE-1;
D O I：
10.1002/sca.21040
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low-density lipoprotein (Ox-LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox-LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical imaging and force measurement functions of atomic force microscopy (AFM), we found that Ox-LDL can transiently weaken the integrity of endothelium by impairing cell spreading of endothelial cells and decrease the attachment of irrelevant blood cells to endothelium by impairing the nonspecific adhesion property of endothelial cells. The AFM-based data provide important information for understanding the effects of Ox-LDL on endothelial cells or during atherogenesis. SCANNING 35: 119-126, 2013. (c) 2012 Wiley Periodicals, Inc.
引用
收藏
页码：119 / 126
页数：8
相关论文
共 30 条
  • [11] Sonodynamic Effects of Hematoporphyrin Monomethyl Ether on CNE-2 Cells Detected by Atomic Force Microscopy
    Jin, Hua
    Zhong, Xing
    Wang, Zhiyong
    Huang, Xun
    Ye, Hongyan
    Ma, Shuyuan
    Chen, Yong
    Cai, Jiye
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2011, 112 (01) : 169 - 178
  • [12] MODIFIED LOW-DENSITY-LIPOPROTEIN AND ITS CONSTITUENTS AUGMENT CYTOKINE-ACTIVATED VASCULAR CELL-ADHESION MOLECULE-1 GENE-EXPRESSION IN HUMAN VASCULAR ENDOTHELIAL-CELLS
    KHAN, BV
    PARTHASARATHY, SS
    ALEXANDER, RW
    MEDFORD, RM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (03) : 1262 - 1270
  • [13] An improved anion-exchange high-performance liquid chromatography method for measuring oxidized form of LDLs in human plasma
    Kitano, Soichi
    Higashimoto, Yuichiro
    Harada, Shoji
    Sano, Masaru
    Kurata, Tsunehiko
    Yamaguchi, Yu
    Kunitomo, Masaru
    Haginaka, Jun
    Yamagishi, Sho-ichi
    [J]. ANNALS OF CLINICAL BIOCHEMISTRY, 2010, 47 : 460 - 466
  • [14] Current understanding of the role of B cell subsets and intimal and adventitial B cells in atherosclerosis
    Kyaw, Tin
    Tipping, Peter
    Toh, Ban-Hock
    Bobik, Alex
    [J]. CURRENT OPINION IN LIPIDOLOGY, 2011, 22 (05) : 373 - 379
  • [15] Adaptive immunity in atherosclerosis: mechanisms and future therapeutic targets
    Lahoute, Charlotte
    Herbin, Olivier
    Mallat, Ziad
    Tedgui, Alain
    [J]. NATURE REVIEWS CARDIOLOGY, 2011, 8 (06) : 348 - 358
  • [16] Progress and challenges in translating the biology of atherosclerosis
    Libby, Peter
    Ridker, Paul M.
    Hansson, Goran K.
    [J]. NATURE, 2011, 473 (7347) : 317 - 325
  • [17] Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells
    Mathur, AB
    Truskey, GA
    Reichert, WM
    [J]. BIOPHYSICAL JOURNAL, 2000, 78 (04) : 1725 - 1735
  • [18] Atomic force microscopy: a nanoscopic window on the cell surface
    Mueller, Daniel J.
    Dufrene, Yves F.
    [J]. TRENDS IN CELL BIOLOGY, 2011, 21 (08) : 461 - 469
  • [19] PARTHASARATHY S, 1992, ANNU REV MED, V43, P219, DOI 10.1146/annurev.me.43.020192.001251
  • [20] Atherosclerosis in psoriatic arthritis
    Ramonda, R.
    Lo Nigro, A.
    Modesti, V.
    Nalotto, L.
    Musacchio, E.
    Iaccarino, L.
    Punzi, L.
    Doria, A.
    [J]. AUTOIMMUNITY REVIEWS, 2011, 10 (12) : 773 - 778
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