AFM Investigation on Ox-LDL-Induced Changes in Cell Spreading and Cell-Surface Adhesion Property of Endothelial Cells

被引：7
作者：
Shao, Wenxiang [1 ,2 ]
Jin, Hua [3 ]
Huang, Jie [2 ]
Qiu, Bin [1 ]
Xia, Ruihua [4 ]
Deng, Zeyuan [1 ]
Cai, Jiye [3 ]
Chen, Yong [1 ,2 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Peoples R China
[2] Nanchang Univ, Inst Adv Study, Nanchang, Peoples R China
[3] Jinan Univ, Dept Chem, Guangzhou, Guangdong, Peoples R China
[4] Jinan Univ, Dept Elect Informat Engn, Nanchang, Peoples R China
来源：
SCANNING | 2013年 / 35卷 / 02期
基金：
中国国家自然科学基金;
关键词：
atherosclerosis; oxidized low-density lipoprotein (Ox-LDL); human umbilical vein endothelial cells (HUVECs); atomic force microscopy (AFM); cell spreading; adhesive force; LOW-DENSITY-LIPOPROTEIN; ATOMIC-FORCE MICROSCOPY; OXIDIZED-LDL; ATHEROSCLEROSIS; PATHOGENESIS; EXPRESSION; PLASMA; ANTIOXIDANTS; DYSFUNCTION; MOLECULE-1;
D O I：
10.1002/sca.21040
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low-density lipoprotein (Ox-LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox-LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical imaging and force measurement functions of atomic force microscopy (AFM), we found that Ox-LDL can transiently weaken the integrity of endothelium by impairing cell spreading of endothelial cells and decrease the attachment of irrelevant blood cells to endothelium by impairing the nonspecific adhesion property of endothelial cells. The AFM-based data provide important information for understanding the effects of Ox-LDL on endothelial cells or during atherogenesis. SCANNING 35: 119-126, 2013. (c) 2012 Wiley Periodicals, Inc.
引用
收藏
页码：119 / 126
页数：8
相关论文
共 30 条
  • [1] Oxidized-LDL induce morphological changes and increase stiffness of endothelial cells
    Chouinard, Julie A.
    Grenier, Guillaume
    Khalil, Abdelouahed
    Vermette, Patrick
    [J]. EXPERIMENTAL CELL RESEARCH, 2008, 314 (16) : 3007 - 3016
  • [2] AFM functional imaging on vascular endothelial cells
    Chtcheglova, Lilia A.
    Wildling, Linda
    Waschke, Jens
    Drenckhahn, Detlev
    Hinterdorfer, Peter
    [J]. JOURNAL OF MOLECULAR RECOGNITION, 2010, 23 (06) : 589 - 596
  • [3] Antioxidants inhibit the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 induced by oxidized LDL on human umbilical vein endothelial cells
    Cominacini, L
    Garbin, U
    Fratta Pasini, A
    Davoli, A
    Campagnola, M
    Contessi, GB
    Pastorino, AM
    LoCascio, V
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (1-2) : 117 - 127
  • [4] Association between circulating oxidised low-density lipoprotein and fibrocalcific remodelling of the aortic valve in aortic stenosis
    Cote, C.
    Pibarot, P.
    Despres, J-P
    Mohty, D.
    Cartier, A.
    Arsenault, B. J.
    Couture, C.
    Mathieu, P.
    [J]. HEART, 2008, 94 (09) : 1175 - 1180
  • [5] Clinical applications of circulating oxidized low-density lipoprotein biomarkers in cardiovascular disease
    Fraley, Alexander E.
    Tsimikas, Sotirios
    [J]. CURRENT OPINION IN LIPIDOLOGY, 2006, 17 (05) : 502 - 509
  • [6] Dual effect of oxidized LDL on cell cycle in human endothelial cells through oxidative stress
    Galle, J
    Heinloth, A
    Wanner, C
    Heermeier, K
    [J]. KIDNEY INTERNATIONAL, 2001, 59 : S120 - S123
  • [7] Cardiovascular Risk and Endothelial Dysfunction: The Preferential Route for Atherosclerosis
    Grassi, Davide
    Desideri, Giovambattista
    Ferri, Claudio
    [J]. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2011, 12 (09) : 1343 - 1353
  • [8] In vitro interactions of oxidatively modified LDL with type I, II, III, IV and V collagen, laminin, fibronectin, and poly-D-lysine
    Greilberger, J
    Schmut, O
    Jurgens, G
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (11) : 2721 - 2728
  • [9] Oxidants and antioxidants in the pathogenesis of atherosclerosis: implications for the oxidized low density lipoprotein hypothesis
    Heinecke, JW
    [J]. ATHEROSCLEROSIS, 1998, 141 (01) : 1 - 15
  • [10] Measurement of plasma oxidized low-density lipoprotein and its clinical implications
    Itabe, Hiroyuki
    Ueda, Makiko
    [J]. JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS, 2007, 14 (01) : 1 - 11
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