Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy

被引：11

作者：

Li, Hsing-Yuan ^{[1
,2
]}

Tseng, Shing-Hua ^{[1
]}

Cheng, Tsai-Mu ^{[1
,3
,4
]}

Chu, Hsueh-Liang ^{[1
]}

Lu, Yu-Ning ^{[1
]}

Wang, Fang-Yu ^{[1
]}

Tsai, Li-Yun ^{[5
]}

Shieh, Juo-Yu ^{[6
]}

Yang, Jyh-Yuan ^{[6
]}

Juan, Chien-Chang

Tu, Lung-Chen ^{[1
,7
]}

Chang, Chia-Ching ^{[1
,8
]}

机构：

[1] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu, Taiwan

[2] Natl Taiwan Univ Hosp, Dept Pediat, Hsinchu Branch, Hsinchu, Taiwan

[3] Taipei Med Univ, PhD Program Translat Med, Taipei, Taiwan

[4] Acad Sinica, Taipei, Taiwan

[5] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

[6] Ctr Dis Control, Res & Diagnost Ctr, Dept Hlth, Taipei, Taiwan

[7] Mackay Mem Hosp, Dept Plast Surg, Taipei, Taiwan

[8] Acad Sinica, Inst Phys, Taipei, Taiwan

来源：

NANOTECHNOLOGY | 2013年 / 24卷 / 28期

关键词：

REVERSE TRANSCRIPTION-PCR; CENTRAL-NERVOUS-SYSTEM; MOUTH-DISEASE; GOLD NANOPARTICLES; COMPLETE GENOME; INFECTION; OUTBREAK; MONOLAYERS; EPIDEMIC; TAIWAN;

D O I：

10.1088/0957-4484/24/28/285102

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 mu 1 reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.

引用

页数：9

共 34 条

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