共 5 条
Biomimetic fabrication and freeze-casting of collagen-apatite hydrogels for bone tissue engineering
被引:0
作者:
Xia, Zengmin
[1
]
Wei, Mei
[1
]
机构:
[1] Univ Connecticut, Dept Mat Sci & Engn, Storrs, CT 06269 USA
来源:
2014 40TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC)
|
2014年
关键词:
collagen;
apatite;
biomimetic;
hydrogels;
freeze-casting;
D O I:
暂无
中图分类号:
R318 [生物医学工程];
学科分类号:
0831 ;
摘要:
Collagen-apatite (Col-Ap) scaffolds have been widely employed for bone tissue engineering. In the current study, we fabricated Col-Ap hydrogels with a biomimetic structure and freeze-dried Col-Ap hydrogels with a hierarchical lamellar structure. Col-Ap hydrogel was prepared using a biomimetic approach combining self-assembling of collagen molecules with simultaneous mineralization in a modified simulated body fluid (m-SBF). A novel two-temperature process involving gelation at 25 degrees C and then 40 degrees C was applied to increase the diameter of mineralized collagen fibers. Col-Ap hydrogel consisting of collagen bundles reinforced by apatite nano-particles was further subjected to self-compression and controlled freeze-casting. The freeze-dried hydrogel exhibits a hierarchical lamellar structure consisting of co-aligned macro and micro pores. Moreover, this novel lamellar structured scaffold supports the attachment and spreading of MC3T3-E1osteoblasts. Therefore, owing to the biomimetic composition, hierarchical lamellar structure and good biocompatibility of this novel freeze-dried Col-Ap hydrogel, it has great potential to be used in bone tissue engineering applications.
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