Biomimetic fabrication and freeze-casting of collagen-apatite hydrogels for bone tissue engineering

被引:0
作者
Xia, Zengmin [1 ]
Wei, Mei [1 ]
机构
[1] Univ Connecticut, Dept Mat Sci & Engn, Storrs, CT 06269 USA
来源
2014 40TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC) | 2014年
关键词
collagen; apatite; biomimetic; hydrogels; freeze-casting;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Collagen-apatite (Col-Ap) scaffolds have been widely employed for bone tissue engineering. In the current study, we fabricated Col-Ap hydrogels with a biomimetic structure and freeze-dried Col-Ap hydrogels with a hierarchical lamellar structure. Col-Ap hydrogel was prepared using a biomimetic approach combining self-assembling of collagen molecules with simultaneous mineralization in a modified simulated body fluid (m-SBF). A novel two-temperature process involving gelation at 25 degrees C and then 40 degrees C was applied to increase the diameter of mineralized collagen fibers. Col-Ap hydrogel consisting of collagen bundles reinforced by apatite nano-particles was further subjected to self-compression and controlled freeze-casting. The freeze-dried hydrogel exhibits a hierarchical lamellar structure consisting of co-aligned macro and micro pores. Moreover, this novel lamellar structured scaffold supports the attachment and spreading of MC3T3-E1osteoblasts. Therefore, owing to the biomimetic composition, hierarchical lamellar structure and good biocompatibility of this novel freeze-dried Col-Ap hydrogel, it has great potential to be used in bone tissue engineering applications.
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页数:2
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共 5 条
[1]   Porous three-dimensional scaffolds made of mineralised collagen:: Preparation and properties of a biomimetic nanocomposite material for tissue engineering of bone [J].
Gelinsky, M. ;
Welzel, P. B. ;
Simon, P. ;
Bernhardt, A. ;
Koenig, U. .
CHEMICAL ENGINEERING JOURNAL, 2008, 137 (01) :84-96
[2]   Modular Design in Natural and Biomimetic Soft Materials [J].
Kushner, Aaron M. ;
Guan, Zhibin .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (39) :9026-9057
[3]   Synthesis of Dense Collagen/Apatite Composites Using a Biomimetic Method [J].
Qu, Haibo ;
Xia, Zengmin ;
Knecht, David A. ;
Wei, Mei .
JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 2008, 91 (10) :3211-3215
[4]   Collagen-apatite nanocomposite membranes for guided bone regeneration [J].
Song, Ju-Ha ;
Kim, Hyoun-Ee ;
Kim, Hae-Won .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2007, 83B (01) :248-257
[5]   A biomimetic collagen-apatite scaffold with a multi-level lamellar structure for bone tissue engineering [J].
Xia, Z. ;
Villa, M. M. ;
Wei, M. .
JOURNAL OF MATERIALS CHEMISTRY B, 2014, 2 (14) :1998-2007