In vitro transcription systems from cultured cells and fat-body tissue of the silkworm, Bombyx mori

被引：4

作者：

Mine, E ^{[1
]}

Sakurai, H ^{[1
]}

Izumi, S ^{[1
]}

Tomino, S ^{[1
]}

机构：

[1] TOKYO METROPOLITAN UNIV,DEPT BIOL,HIACHIOJI,TOKYO 19203,JAPAN

来源：

MOLECULAR BIOTECHNOLOGY | 1997年 / 8卷 / 01期

关键词：

in vitro transcription; whole-cell extract; nuclear extract; fat body; BmN cell; TC-100; medium; S1 nuclease protection assay; primer extension analysis; Bombyx mori; artificial diet for B-mori; SP1; gene; template DNA; alpha-amanitin; conditions for cell-free transcription;

D O I：

10.1007/BF02762341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes the development of cell-free transcription systems from the cultured cells and fat body tissues of a Lepidpteran insect, the silkworm, Bombyx mori. Detailed protocols are provided for the culture of a B. mori cell line, rearing larvae, preparation of whole cell as well as nuclear extract and conditions for in vitro transcription of cloned plasma protein gene templates.

引用

页码：79 / 88

页数：10

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