Algoriphagus confluentis sp nov., isolated from the junction between the ocean and a freshwater lake

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-2(T), was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HJM-2(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HJM-2(T) belonged to the genus Algoriphagus, clustering coherently with the type strain of A. taiwanensis. Strain HJM-2(T) exhibited 16S rRNA gene sequence similarities of 98.6 and 97.2 % to A. taiwanensis CC-RR-82(T) and A. boseongensis BS-R1(T), respectively, and 92.7-96.7 % to the type strains of the other species of the genus Algoriphagus. Strain HJM-2(T) contained MK-7 as the predominant menaquinone and iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and iso-C-17:0 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-2(T) were phosphatidylcholine, phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-2(T) was 45 mol% and mean DNA DNA relatedness values with the type strains of A. taiwanensis and A. boseongensis were 10-19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain HJM-2(T) is separated from recognized species of the genus Algoriphagus. On the basis of the data presented, strain HJM-2(T) represents a novel species of the genus Algoriphagus, for which the name Algoriphagus confluentis sp. nov. is proposed. The type strain is HJM-2(T) (=KCTC 42704(T)=NBRC 111222(T)).