Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats

被引：27
作者：
Ji, Yuanyuan [1 ]
Zhang, Aijun [2 ]
Chen, Xiaobin [2 ]
Che, Xiaoxia [2 ]
Zhou, Kai [2 ]
Wang, Zhidong [3 ]
机构：
[1] Xi An Jiao Tong Univ, Affiliated Hosp 2, Ctr Sci Res, Xian 710004, Peoples R China
[2] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Pharm, Xian 710004, Peoples R China
[3] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept VIP Gen Surg, Xian 710004, Peoples R China
来源：
ACTA PHARMACEUTICA SINICA B | 2016年 / 6卷 / 02期
关键词：
Sodium humate; Wound healing; Transforming growth factor-beta; Smad; Rat; ENDOTHELIAL GROWTH-FACTOR; ANTIOXIDANT PROPERTIES; TOPICAL APPLICATION; HUMIC SUBSTANCES; FULVIC-ACID; FACTOR-BETA; REGENERATION; FIBROBLASTS; COLLAGEN; REPAIR;
D O I：
10.1016/j.apsb.2016.01.009
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Sodium humate (HA-Na) has been topically used as a wound healing and anti-inflammatory agent in folk medicine. In the present study, HA-Na was investigated for cutaneous wound healing in Sprague-Dawley rats. HA-Na solution (1.0%, w/v) was topically administered to rats undergoing excision wound models. Healing was assessed with a recombinant bovine basic fibroblast growth factor for external use as positive control. Wound healing rates were calculated on Day 3, 6, 9, 14 and 21 after injury, and tissues were also harvested after the same intervals for histological analysis. In addition, tissue hydroxyproline levels were measured. Furthermore, mRNA levels and protein expressions of transforming growth factor-beta 1, 2, 3 (TGF-beta 1, 2, 3) were determined by RT-PCR and western blot. Protein expression levels of Smad-2, -3, -4 and -7 were also detected by western blot. Our study demonstrates that HA-Na has the capacity to promote wound healing in rats via accelerated wound contraction and increased hydroxyproline content. More importantly, these wound healing effects of HA-Na might be mediated through the TGF-beta/Smad signaling pathway. HA-Na may be an effective agent for enhanced wound healing. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
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收藏
页码：132 / 140
页数：9
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