Persistent Spike-specific T cell immunity despite antibody reduction after 3 months from SARS-CoV-2 BNT162b2-mRNA vaccine

被引：33

作者：

Agrati, Chiara ^{[1
]}

Castilletti, Concetta ^{[1
]}

Goletti, Delia ^{[1
]}

Sacchi, Alessandra ^{[1
]}

Bordoni, Veronica ^{[1
]}

Mariotti, Davide ^{[1
]}

Notari, Stefania ^{[1
]}

Matusali, Giulia ^{[1
]}

Meschi, Silvia ^{[1
]}

Petrone, Linda ^{[1
]}

Aiello, Alessandra ^{[1
]}

Fard, Saeid Najafi ^{[1
]}

Farroni, Chiara ^{[1
]}

Colavita, Francesca ^{[1
]}

Lapa, Daniele ^{[1
]}

Leone, Sara ^{[1
]}

Agresta, Alessandro ^{[1
]}

Capobianchi, Maria ^{[1
]}

Ippolito, Giuseppe ^{[1
]}

Vaia, Francesco ^{[1
]}

Puro, Vincenzo ^{[1
]}

机构：

[1] INMI L Spallanzani IRCCS, Via Portuense 292, I-00149 Rome, Italy

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

关键词：

D O I：

10.1038/s41598-022-07741-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vaccine is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive scientific effort worldwide resulted in the rapid development of effective vaccines. This work aimed to define the dynamics and persistence of humoral and cell-mediated immune response in Health Care Workers who received a two-dose BNT162b2-mRNA vaccination. Serological response was evaluated by quantifying anti-RBD and neutralizing antibodies while cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-gamma, TNF-alpha, IL-2) produced in response to Spike peptides. BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune response against Spike in all HCW early after the second dose. After 12 weeks from vaccination, the titer of anti-RBD antibodies as well as their neutralization function decreased while the Spike-specific T-cells persisted at the same level as soon after vaccine boost. Of note, a correlation between cellular and humoral response persevered, suggesting the persistence of a coordinated immune response. The long lasting cell-mediated immune response after 3 months from vaccination highlight its importance in the maintaining of specific immunity able to expand again to fight eventual new antigen encountering.

引用

页数：5

共 15 条

[1] Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine [J].

Agrati, Chiara ;

Castilletti, Concetta ;

Goletti, Delia ;

Meschi, Silvia ;

Sacchi, Alessandra ;

Matusali, Giulia ;

Bordoni, Veronica ;

Petrone, Linda ;

Lapa, Daniele ;

Notari, Stefania ;

Vanini, Valentina ;

Colavita, Francesca ;

Aiello, Alessandra ;

Agresta, Alessandro ;

Farroni, Chiara ;

Grassi, Germana ;

Leone, Sara ;

Vaia, Francesco ;

Capobianchi, Maria Rosaria ;

Ippolito, Giuseppe ;

Puro, Vincenzo .

MICROORGANISMS, 2021, 9 (06)

[2] Insights into human CD8+ T-cell memory using the yellow fever and smallpox vaccines [J].

Ahmed, Rafi ;

Akondy, Rama S. .

IMMUNOLOGY AND CELL BIOLOGY, 2011, 89 (03) :340-345

[3] Spike is the most recognized antigen in the whole-blood platform in both acute and convalescent COVID-19 patients [J].

Aiello, Alessandra ;

Fard, Saeid Najafi ;

Petruccioli, Elisa ;

Petrone, Linda ;

Vanini, Valentina ;

Farroni, Chiara ;

Cuzzi, Gilda ;

Navarra, Assunta ;

Gualano, Gina ;

Mosti, Silvia ;

Pierelli, Luca ;

Nicastri, Emanuele ;

Goletti, Delia .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2021, 106 :338-347

[4]

Angyal A, SSRN Electron. J., DOI [10.2139/ssrn.3812375, DOI 10.2139/SSRN.3812375]

[5] Immunological memory to SARS-CoV-2 assessed for up to 8 months after infection [J].

Dan, Jennifer M. ;

Mateus, Jose ;

Kato, Yu ;

Hastie, Kathryn M. ;

Yu, Esther Dawen ;

Faliti, Caterina E. ;

Grifoni, Alba ;

Ramirez, Sydney, I ;

Haupt, Sonya ;

Frazier, April ;

Nakao, Catherine ;

Rayaprolu, Vamseedhar ;

Rawlings, Stephen A. ;

Peters, Bjoern ;

Krammer, Florian ;

Simon, Viviana ;

Saphire, Erica Ollmann ;

Smith, Davey M. ;

Weiskopf, Daniela ;

Sette, Alessandro ;

Crotty, Shane .

SCIENCE, 2021, 371 (6529) :587-+

[6]

Goel Rishi R, 2021, bioRxiv, DOI 10.1101/2021.08.23.457229

[7] Lasting antibody and T cell responses to SARS-CoV-2 in COVID-19 patients three months after infection [J].

Jiang, Xiao-Lin ;

Wang, Guo-Lin ;

Zhao, Xiang-Na ;

Yan, Fei-Hu ;

Yao, Lin ;

Kou, Zeng-Qiang ;

Ji, Sheng-Xiang ;

Zhang, Xiao-Li ;

Li, Cun-Bao ;

Duan, Li-Jun ;

Li, Yan ;

Zhang, Yu-Wen ;

Duan, Qing ;

Wang, Tie-Cheng ;

Li, En-Tao ;

Wei, Xiao ;

Wang, Qing-Yang ;

Wang, Xue-Feng ;

Sun, Wei-Yang ;

Gao, Yu-Wei ;

Kang, Dian-Min ;

Zhang, Ji-Yan ;

Ma, Mai-Juan .

NATURE COMMUNICATIONS, 2021, 12 (01)

[8] Performance evaluation of Abbott ARCHITECT SARS-CoV-2 IgG immunoassay in comparison with indirect immunofluorescence and virus microneutralization test [J].

Meschi, Silvia ;

Colavita, Francesca ;

Bordi, Licia ;

Matusali, Giulia ;

Lapa, Daniele ;

Amendola, Alessandra ;

Vairo, Francesco ;

Ippolito, Giuseppe ;

Capobianchi, Maria Rosaria ;

Castilletti, Concetta .

JOURNAL OF CLINICAL VIROLOGY, 2020, 129

[9] Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity [J].

Moderbacher, Carolyn Rydyznski ;

Ramirez, Sydney, I ;

Dan, Jennifer M. ;

Grifoni, Alba ;

Hastie, Kathryn M. ;

Weiskopf, Daniela ;

Belanger, Simon ;

Abbott, Robert K. ;

Kim, Christina ;

Choi, Jinyong ;

Kato, Yu ;

Crotty, Eleanor G. ;

Kim, Cheryl ;

Rawlings, Stephen A. ;

Mateus, Jose ;

Tse, Long Ping Victor ;

Frazier, April ;

Baric, Ralph ;

Peters, Bjoern ;

Greenbaum, Jason ;

Saphire, Erica Ollmann ;

Smith, Davey M. ;

Sette, Alessandro ;

Crotty, Shane .

CELL, 2020, 183 (04) :996-+

[10]

Peng YC, 2020, NAT IMMUNOL, V21, P1336, DOI [10.1038/s41590-020-0782-6, 10.1101/2020.06.05.134551]

← 1 2 →