High Expression of Macrophage Migration Inhibitory Factor in the Murine Lacrimal Gland

被引:0
|
作者
Park, Choul Yong [2 ]
Shin, Young Joo
Lekhanont, Kaevalin
Zhang, Cheng
Lee, Woo-Seok
Cano, Marisol
Bucala, Richard [3 ]
Chuck, Roy S. [1 ]
机构
[1] Johns Hopkins Univ, Wilmer Ophthalmol Inst, Dept Ophthalmol, Baltimore, MD 21117 USA
[2] Dongguk Univ, Sch Med, Dept Ophthalmol, Ilsan, South Korea
[3] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA
基金
美国国家卫生研究院;
关键词
MIF; macrophage; inhibition; lacrimal gland; dry eye; ocular surface; inflammation; dysfunctional tear; mouse; DRY-EYE; FACTOR MIF; INCREASE; CYTOKINE; DISEASE; FLUID; SERA;
D O I
暂无
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription-polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of P-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusion: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.
引用
收藏
页码:187 / 191
页数:5
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