Analysis of minimal residual disease by Ig/TCR gene rearrangements: guidelines for interpretation of real-time quantitative PCR data

被引：576

作者：

van der Velden, V. H. J.

Cazzaniga, G.

Schrauder, A.

Hancock, J.

Bader, P.

Panzer-Grumayer, E. R.

Flohr, T.

Sutton, R.

Cave, H.

Madsen, H. O.

Cayuela, J. M.

Trka, J.

Eckert, C.

Foroni, L.

zur Stadt, U.

Beldjord, K.

Raff, T.

van der Schoot, C. E.

van Dongen, J. J. M.

机构：

[1] Erasmus MC, Dept Immunol, NL-3015 GE Rotterdam, Netherlands

[2] Univ Milan, Ctr Ric Tettamanti, Monza, Italy

[3] Univ Hosp Schleswig Holstein, Dept Pediat, Kiel, Germany

[4] Univ Bristol, Dept Cellular & Mol Med, Bristol BS8 1TH, Avon, England

[5] Klinikum Johann Wolfgang Goethe Univ, Frankfurt, Germany

[6] Childrens Canc Res Inst, Vienna, Austria

[7] St Anna Childrens Hosp, Vienna, Austria

[8] Heidelberg Univ, Inst Human Genet, Heidelberg, Germany

[9] Univ NSW, Childrens Canc Inst Australia Med Res, Sydney, NSW, Australia

[10] Hop Robert Debre, Biochim Genet Lab, F-75019 Paris, France

[11] Rigshosp, Dept Clin Immunol, DK-2100 Copenhagen, Denmark

[12] Hop St Louis, Cent Hematol Lab, Paris, France

[13] Charles Univ Prague, Dept Paediat Haematol Oncol, Sch Med 2, Prague, Czech Republic

[14] Charite Med Ctr CVK, Dept Paediat Oncol & Hematol, Berlin, Germany

[15] UCL Royal Free & Univ Coll, Sch Med, Dept Acad Haematol, London, England

[16] Univ Med Ctr Hamburg Eppendorf, Dept Pediat Hematol & Oncol, Hamburg, Germany

[17] Hop Necker Enfants Malad, Hematol Lab, Paris, France

[18] Univ Klinikum Schleswig Holstein, Med Klin 2, Kiel, Germany

[19] Sanquin, Dept Expt Immunohematol, Amsterdam, Netherlands

来源：

LEUKEMIA | 2007年 / 21卷 / 04期

关键词：

MRD; ALL; Ig/; TCR; RQ-PCR; guidelines;

D O I：

10.1038/sj.leu.2404586

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Most modern treatment protocols for acute lymphoblastic leukaemia (ALL) include the analysis of minimal residual disease (MRD). To ensure comparable MRD results between different MRD-polymerase chain reaction (PCR) laboratories, standardization and quality control are essential. The European Study Group on MRD detection in ALL (ESG-MRD-ALL), consisting of 30 MRD-PCR laboratories worldwide, has developed guidelines for the interpretation of real-time quantitative PCR-based MRD data. The application of these guidelines ensures identical interpretation of MRD data between different laboratories of the same MRD-based clinical protocol. Furthermore, the ESG-MRD-ALL guidelines will facilitate the comparison of MRD data obtained in different treatment protocols, including those with new drugs.

引用

页码：604 / 611

页数：8

共 23 条

[21] T-lymphocytes in bone marrow samples of children with acute lymphoblastic leukemia during and after chemotherapy might hamper PCR-based minimal residual disease studies [J].