Enhancer of zeste homolog 2 enhances the migration and chemotaxis of dental mesenchymal stem cells

被引：4

作者：

Ma, Huarui ^{[1
]}

Duan, Shaoyu ^{[2
]}

Yan, Fei ^{[3
,4
]}

Yang, Haoqing ^{[5
]}

Cao, Yangyang ^{[5
]}

Ge, Lihua ^{[5
]}

Gao, Runtao ^{[1
]}

机构：

[1] Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing 100050, Peoples R China

[2] Beijing Elect Power Hosp, Dept Stomatol, Beijing, Peoples R China

[3] Cent South Univ, Xiangya Stomatol Hosp, Changsha, Hunan, Peoples R China

[4] Cent South Univ, Sch Stomatol, Changsha, Hunan, Peoples R China

[5] Capital Med Univ, Sch Stomatol, 4 Tian Tan Xi Li, Beijing, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2020年 / 48卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Chemotaxis; enhancer of zeste homolog 2; human dental tissue-derived mesenchymal stem cells; migration; C-X-C motif chemokine; homing; BONE-MARROW; PULP INFLAMMATION; APICAL PAPILLA; STROMAL CELLS; EZH2; EXPRESSION; PROLIFERATION; INVOLVEMENT; FRACTALKINE; METASTASIS;

D O I：

10.1177/0300060519882149

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective To investigate the function of enhancer of zeste homolog 2 (EZH2) in the migration and chemotaxis of human dental tissue-derived mesenchymal stem cells. Methods The expression of EZH2, C-X-C motif chemokine ligand 11 (CXCL11), CXCL16, and CXCR1 in stem cells from the apical papilla (SCAPs) was determined by real-time reverse transcription PCR and western blotting. The effects of EZH2 on the homing of SCAPs and the effects of EZH2-overexpressing SCAP culture supernatant on periodontal ligament stem cells (PDLSCs) were tested by scratch migration assays and transwell chemotaxis assays. Results EZH2 overexpression significantly enhanced the migration and chemotaxis of SCAPs and upregulated the expression of CXCL11, CXCL16, and CXCR1 in SCAPs. EZH2 depletion had the opposite effect, impairing the migration and chemotaxis of SCAPs and downregulating the expression of CXCL11, CXCL16, and CXCR1. The culture supernatant of EZH2-overexpressing SCAPs advanced the migration and chemotaxis of PDLSCs. Conclusions EZH2 evidently promoted the migration and chemotaxis of SCAPs by upregulating the expression of CXCL11, CXCL16, and CXCR1. Moreover, EZH2-overexpressing SCAPs enhanced the homing, migration, and chemotaxis of PDLSCs via paracrine signaling.

引用

页数：16

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