Sequencing and analysis of a Mycoplasma gallisepticum A5969 chromosome region containing the S10 and rrn23-5 operons

被引：0

作者：

Skamrov, AV ^{[1
]}

Gol'dman, MA ^{[1
]}

Feoktistova, ES ^{[1
]}

Bibilashvili, RS ^{[1
]}

机构：

[1] Minist Hlth Russian Federat, Cardiol Res Ctr, Inst Expt Cardiol, Moscow 121552, Russia

来源：

MOLECULAR BIOLOGY | 2000年 / 34卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Mycoplasma gallisepticum; gene; operon; ribosomal proteins; rRNA; mapping;

D O I：

10.1007/BF02759662

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 20,115-nt region of the Mycoplasma gallisepticum A5959 genome was sequenced (GenBank accession no. AF036708). The region contains the rrn23-5 and S10 operons, the lactate dehydrogenase gene, and two open reading frames (ORF293 and ORF129/ORF171) coding for proteins of unknown function. The rrn23-5 operon includes genes for 23S and 5S rRNAs. The S10 operon includes genes for 20 ribosomal proteins, SecY transport protein, adenylate kinase, and methionine aminopeptidase, and lacks the infA-rpl36-rps13-rpoA-rpl17 genes found in the S10 operon of M. genitalium, M. pneumoniae, and Bacillus subtilis. The product of M gallisepticum ldh is equally similar to the corresponding proteins of mycoplasmata and B. subtilis but contains only a part of the motif characteristic of the active center of lactate dehydrogenases. The chromosome region adjacent to the sequenced one contains uvrA, nrdE, nrdF, and ptsI.

引用

页码：331 / 336

页数：6

共 13 条

[1] NOVEL ARRANGEMENT OF RIBOSOMAL-RNA GENES IN MYCOPLASMA-GALLISEPTICUM - SEPARATION OF THE 16S-GENE OF ONE SET FROM THE 23S-GENE AND 5S-GENE [J].

CHEN, X ;

FINCH, LR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2876-2878

[2] Malate/lactate dehydrogenase in mollicutes: evidence for a multienzyme protein [J].

Cordwell, SJ ;

Basseal, DJ ;

Pollack, JD ;

HumpherySmith, I .

GENE, 1997, 195 (02) :113-120

[3] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[4] THE MINIMAL GENE COMPLEMENT OF MYCOPLASMA-GENITALIUM [J].

FRASER, CM ;

GOCAYNE, JD ;

WHITE, O ;

ADAMS, MD ;

CLAYTON, RA ;

FLEISCHMANN, RD ;

BULT, CJ ;

KERLAVAGE, AR ;

SUTTON, G ;

KELLEY, JM ;

FRITCHMAN, JL ;

WEIDMAN, JF ;

SMALL, KV ;

SANDUSKY, M ;

FUHRMANN, J ;

NGUYEN, D ;

UTTERBACK, TR ;

SAUDEK, DM ;

PHILLIPS, CA ;

MERRICK, JM ;

TOMB, JF ;

DOUGHERTY, BA ;

BOTT, KF ;

HU, PC ;

LUCIER, TS ;

PETERSON, SN ;

SMITH, HO ;

HUTCHISON, CA ;

VENTER, JC .

SCIENCE, 1995, 270 (5235) :397-403

[5]

GHOSH A, 1977, J MOL BIOL, V166, P337

[6] Comparative analysis of the genomes of the bacteria Mycoplasma pneumoniae and Mycoplasma genitalium [J].

Himmelreich, R ;

Plagens, H ;

Hilbert, H ;

Reiner, B ;

Herrmann, R .

NUCLEIC ACIDS RESEARCH, 1997, 25 (04) :701-712

[7] Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae [J].

Himmelreich, R ;

Hilbert, H ;

Plagens, H ;

Pirkl, E ;

Li, BC ;

Herrmann, R .

NUCLEIC ACIDS RESEARCH, 1996, 24 (22) :4420-4449

[8] Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster [J].

Li, X ;

Lindahl, L ;

Sha, YZ ;

Zengel, JM .

JOURNAL OF BACTERIOLOGY, 1997, 179 (22) :7046-7054

[9] NUCLEOTIDE-SEQUENCE, ORGANIZATION AND CHARACTERIZATION OF THE ATP GENES AND THE ENCODED SUBUNITS OF MYCOPLASMA-GALLISEPTICUM ATPASE [J].

RASMUSSEN, OF ;

SHIRVAN, MH ;

MARGALIT, H ;

CHRISTIANSEN, C ;

ROTTEM, S .

BIOCHEMICAL JOURNAL, 1992, 285 :881-888

[10] Molecular biology and pathogenicity of mycoplasmas [J].

Razin, S ;

Yogev, D ;

Naot, Y .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) :1094-+

← 1 2 →