Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

被引：0
作者：
Thiem, B
Kalinowski, A
机构：
[1] Univ Med Sci, Dept Pharmaceut Sci, PL-61861 Poznan, Poland
[2] Polish Acad Sci, Inst Plant Genet, PL-60479 Poznan, Poland
来源：
ACTA SOCIETATIS BOTANICORUM POLONIAE | 2002年 / 71卷 / 04期
关键词：
Rubus chamaemorus; in vitro culture; shoot culture; protein; two-dimensional electrophoresis;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of plants from in vitro culture. A two-dimensional map of proteins from leaves provides detailed data concerning both polymorphism and protein patterns of this species. This makes it possible to start constructing a protein map of R. chamaemorus. The reasons for qualitative differences are discussed.
引用
收藏
页码：299 / 305
页数：7
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