Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin

被引：60
|
作者：
Wang, Huixiang [1 ]
Liu, Yun [1 ]
Liu, Chengcheng [1 ]
Huang, Jingyu [1 ]
Yang, Pengyuan [1 ]
Liu, Baohong [1 ]
机构：
[1] Fudan Univ, Dept Chem, Inst Biomed Sci, Shanghai 200433, Peoples R China
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2010年 / 12卷 / 02期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Aptamer; Microchip; Thrombin; Electrochemistry; Sensor; ASSEMBLED MICROCHIP; PROTEIN; APTAMER; SURFACE; STRATEGY; GOLD; NANOPARTICLES; PROTEOLYSIS; BIOSENSOR; LIGANDS;
D O I：
10.1016/j.elecom.2009.12.008
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
In this paper, a microchip-based sandwich-type aptasensor is developed for the detection of human thrombin. The SH-aptamer/thrombin/alkaline phosphatase-functionalized aptamer (ALP-aptamer) system was constructed in the microfluidic channels. And the substrate solution containing 4-aminophenyl phosphate (p-APP) was introduced to the microchannels for the end-column electrochemical detection. The on-chip aptasensor has a broad linear response range of 1-100 pM with a detection limit of 1 pM, which shows high sensitivity and specificity. The system was then applied to detect thrombin in human serum sample. Therefore, the on-chip aptasensor has a great promise for detecting and screening ultra-trace levels of biomarkers in the complex matrices. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：258 / 261
页数：4
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