Picosecond transient circular dichroism of the photoreceptor protein of the light-adapted form of Blepharisma japonicum

被引：9
|
作者：
Hache, Francois [1 ]
Khuc, Mai-Thu [1 ]
Brazard, Johanna [2 ]
Plaza, Pascal [2 ]
Martin, Monique M. [2 ]
Checcucci, Giovanni [3 ]
Lenci, Francesco [3 ]
机构：
[1] Ecole Polytech, CNRS, INSERM, Lab Opt & Biosci, F-91128 Palaiseau, France
[2] UPMC, ENS, CNRS, UMR 8640,Dept Chim, F-75005 Paris, France
[3] CNR, Ist Biofis, I-56100 Pisa, Italy
来源：
CHEMICAL PHYSICS LETTERS | 2009年 / 483卷 / 1-3期
关键词：
UP PHOTOPHOBIC RESPONSE; PRIMARY PHOTOPROCESSES; LASER PHOTOLYSIS; ACTION SPECTRA; OXYBLEPHARISMIN; PIGMENT;
D O I：
10.1016/j.cplett.2009.10.059
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
We present a picosecond transient circular dichroism study of OBIP, the putative photoreceptor protein involved in the photophobic response of Blepharisma japonicum. The probe wavelength was chosen at 230 nm. The results are compared to those of the isolated chromophore, OxyBP, in solution. The CD changes in OBIP and OxyBP do not show the same dynamics: OBIP's signal relaxes in a few ps whereas no such decay is obtained for OxyBP. This observation brings support to the formerly evoked existence of a fast photoinduced reaction in the chromoprotein, and demonstrates the implication of local geometrical changes that accompany this process. (C) 2009 Published by Elsevier B. V.
引用
收藏
页码：133 / 137
页数：5
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