Simple and reproducible HPLC-DAD-ESI-MS/MS analysis of alkaloids in Catharanthus roseus roots

被引：38

作者：

Ferreres, Federico ^{[2
]}

Pereira, David M. ^{[1
]}

Valentao, Patricia ^{[1
]}

Oliveira, Jorge M. A. ^{[3
]}

Faria, Joana ^{[1
]}

Gaspar, Luis ^{[1
]}

Sottomayor, Mariana ^{[4
,5
]}

Andrade, Paula B. ^{[1
]}

机构：

[1] Univ Porto, Fac Pharm, REQUIMTE, Dept Pharmacognosy, P-4050047 Oporto, Portugal

[2] CSIC, CEBAS, Dept Food Sci & Technol, Res Grp Qual Safety & Bioact Plant Foods, Murcia 30100, Spain

[3] Univ Porto, Fac Pharm, REQUIMTE FARMA, Serv Farmacol, P-4050047 Oporto, Portugal

[4] Univ Porto, IBMC Inst Biol Mol & Celular, P-4150180 Oporto, Portugal

[5] Univ Porto, Fac Ciencias, Dept Bot, P-4150180 Oporto, Portugal

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 51卷 / 01期

关键词：

Catharanthus roseus; Roots; HPLC-DAD-ESI-MS/MS; Alkaloids; PERFORMANCE LIQUID-CHROMATOGRAPHY; INDOLE ALKALOIDS;

D O I：

10.1016/j.jpba.2009.08.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Catharanthus roseus is one of the most important medicinal plants worldwide. The leaves of this species are the only source of the indolomonoterpenic alkaloids vincristin (leurocristine) and vinblastin (vincaleucoblastine), whose anticancer activity represents powerful therapeutics to many diseases, such as Hodgkin lymphoma. Usually, the remaining plant parts go to waste. Here we describe a phytochemical study on this species roots. Alkaloids in aqueous extracts, the usual form of consumption of this matrix, were studied using HPLC-DAD-ESI-MS/MS, which allowed the identification of 19-S-vindolinine, vindolinine, ajmalicine and an ajmalicine isomer, tabersonine, catharanthine, serpentine and a serpentine isomer. Quantification of the identified compounds revealed that serpentine and its isomer were predominant (64.7%) over the other alkaloids, namely vindolinine and its isomer (23.9%), catharanthine (7.7%) and ajmalicine (3.8%). The used procedure revealed to be simple, sensitive and reproducible. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：65 / 69

页数：5

共 13 条

[1]

BRUNETON J, 1999, PAHRMACOGNOSIE PHYTO

[2] Molecular cloning and characterization of a vacuolar class III peroxidase involved in the metabolism of anticancer alkaloids in Catharanthus roseus [J].

Costa, Maria Manuela R. ;

Hilliou, Frederique ;

Duarte, Patricia ;

Pereira, Luis Gustavo ;

Almeida, Iolanda ;

Leech, Mark ;

Memelink, Johan ;

Barcelo, Alfonso Ros ;

Sottomayor, Mariana .

PLANT PHYSIOLOGY, 2008, 146 (02) :403-417

[3] BIOSYNTHESIS OF INDOLE ALKALOIDS - DEVELOPMENTAL REGULATION OF THE BIOSYNTHETIC-PATHWAY FROM TABERSONINE TO VINDOLINE IN CATHARANTHUS-ROSEUS [J].

DELUCA, V ;

BALSEVICH, J ;

TYLER, RT ;

EILERT, U ;

PANCHUK, BD ;

KURZ, WGW .

JOURNAL OF PLANT PHYSIOLOGY, 1986, 125 (1-2) :147-156

[4] RADIOIMMUNOASSAY FOR THE QUANTITATIVE-DETERMINATION OF CATHARANTHINE [J].

DEUSNEUMANN, B ;

STOCKIGT, J ;

ZENK, MH .

PLANTA MEDICA, 1987, (02) :184-188

[5]

Digvijay S, 2004, J AOAC INT, V87, P1287

[6]

DONG JG, 1995, PHYTOCHEMISTRY, V40, P1821, DOI 10.1016/0031-9422(95)00627-J

[7] Analysis of Catharanthus roseus alkaloids by HPLC [J].

Hisiger S. ;

Jolicoeur M. .

Phytochemistry Reviews, 2007, 6 (2-3) :207-234

[8]

Kuehne M.E., 1990, The Alkaloids, V37, P77

[9]

ROSS IA, 2003, MED PLANTS WORLD, V1, P175

[10] Validation of high-performance liquid chromatography methods for pharmaceutical analysis - Understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International Conference on Harmonization [J].

Shabir, GA .

JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :57-66

← 1 2 →