Transcriptional Mechanism of the β4-Galactosyltransferase 4 Gene in SW480 Human Colon Cancer Cell Line

被引：9

作者：

Sugiyama, Atena ^{[1
]}

Fukushima, Naomichi ^{[1
]}

Sato, Takeshi ^{[1
]}

机构：

[1] Nagaoka Univ Technol, Dept Bioengn, Lab Glycobiol, Nagaoka, Niigata 9401188, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2017年 / 40卷 / 05期

关键词：

beta; 4-galactosyltransferase; 4; transcriptional mechanism; colon cancer; transcription factor; Specificity protein I (Spl); HUMAN BETA-1,4-GALACTOSYLTRANSFERASE-V GENE; EXPRESSION LEVELS; FACTOR SP1; IN-VITRO; C-MYC; GLYCOSYLATION; IDENTIFICATION; METASTASIS; CARCINOMA; APOPTOSIS;

D O I：

10.1248/bpb.b17-00064

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Increased expression of beta 4-galactosyltransferase (beta 4GalT) 4 has been shown to be associated with meta-static ability and poor prognosis of colon cancer cells. To solve the up-regulation of beta 4GalT4 in colon cancer cells at transcriptional level, we examined the transcriptional mechanism of the beta 4GalT4 gene in SW480 human colon cancer cell line. Luciferase assay using the deletion constructs revealed that the promoter activity of the beta 4GalT4 gene is associated with the region between nucleotides -122 and -55 relative to the transcriptional start site, which contained one Specificity protein 1 (Spl)-binding site. The mutation into the Spl-binding site resulted in dramatic decreased promoter activity. Meanwhile, ectopic Spl expression stimulated the promoter activity significantly. The present study suggests that the expression of the beta 4GalT4 gene is controlled by Spl, and Spl plays a key role in the activation of the beta 4GalT4 gene in colon cancer cells.

引用

页码：733 / 737

页数：5

共 30 条

[1]

Asada M, 1997, CANCER RES, V57, P1073

[2] Sp1 and the 'hallmarks of cancer' [J].

Beishline, Kate ;

Azizkhan-Clifford, Jane .

FEBS JOURNAL, 2015, 282 (02) :224-258

[3] Tumor β-1,4-galactosyltransferase IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis [J].

Chen, WS ;

Chang, HY ;

Li, CP ;

Liu, JM ;

Huang, TS .

CLINICAL CANCER RESEARCH, 2005, 11 (24) :8615-8622

[4] DEREGULATION OF C-MYC GENE-EXPRESSION IN HUMAN-COLON CARCINOMA IS NOT ACCOMPANIED BY AMPLIFICATION OR REARRANGEMENT OF THE GENE [J].

ERISMAN, MD ;

ROTHBERG, PG ;

DIEHL, RE ;

MORSE, CC ;

SPANDORFER, JM ;

ASTRIN, SM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) :1969-1976

[5]

Furukawa K., 2014, HDB GLYCOSYLTRANSFER, P141

[6] N-acetylglucosaminyltransferase V expression levels regulate cadherin-associated homotypic cell-cell adhesion and intracellular signaling pathways [J].

Guo, HB ;

Lee, I ;

Kamar, M ;

Pierce, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (52) :52412-52424

[7]

Guo HB, 2002, CANCER RES, V62, P6837

[8] Glycosylation defining cancer malignancy: New wine in an old bottle [J].

Hakomori, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (16) :10231-10233

[9] Carbohydrate-mediated cell adhesion in cancer metastasis and angiogenesis [J].

Kannagi, R ;

Izawa, M ;

Koike, T ;

Miyazaki, K ;

Kimura, N .

CANCER SCIENCE, 2004, 95 (05) :377-384

[10] Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma [J].

Kara, Murat ;

Yumrutas, Onder ;

Ozcan, Onder ;

Celik, Ozgur Ilhan ;

Bozgeyik, Esra ;

Bozgeyik, Ibrahim ;

Tasdemir, Sener .

GENE, 2015, 567 (01) :81-86

← 1 2 3 →