Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS

被引：15

作者：

Kassim, Nur Sofiah Abu ^{[1
]}

Gomes, Fabio P. ^{[1
]}

Shaw, Paul Nicholas ^{[1
]}

Hewavitharana, Amitha K. ^{[1
]}

机构：

[1] Univ Queensland, Sch Pharm, St Lucia, Qld 4072, Australia

来源：

BIOANALYSIS | 2016年 / 8卷 / 05期

关键词：

LC-MS/MS; plasma; protein precipitation; saponification; serum; vitamin D; CHROMATOGRAPHY-MASS SPECTROMETRY; DIELS-ALDER DERIVATIZATION; LIQUID-CHROMATOGRAPHY; D METABOLITES; PLASMA; QUANTIFICATION; EXTRACTION; 25-HYDROXYVITAMIN-D3; VALIDATION; 3-SULFATE;

D O I：

10.4155/bio.15.260

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research. Materials & methods: Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector. Results & conclusion: Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.

引用

页码：397 / 411

页数：15

共 31 条

[1] A rapid analytical method for cholecalciferol (vitamin D3) in fortified infant formula, milk and milk powder using Diels-Alder derivatisation and liquid chromatography-tandem mass spectrometric detection [J].

Abernethy, Grant A. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2012, 403 (05) :1433-1440

[2] Vitamin D2 is much less effective than vitamin D3 in humans [J].

Armas, LAG ;

Hollis, BW ;

Heaney, RP .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (11) :5387-5391

[3] Metabolic profiling of major vitamin D metabolites using Diels-Alder derivatization and ultra-performance liquid chromatography-tandem mass spectrometry [J].

Aronov, Pavel A. ;

Hall, Laura M. ;

Dettmer, Katja ;

Stephensen, Charles B. ;

Hammock, Bruce D. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (05) :1917-1930

[4] 25-HYDROXYVITAMIN-D3 3-SULFATE IS A MAJOR CIRCULATING FORM OF VITAMIN-D IN MAN [J].

AXELSON, M .

FEBS LETTERS, 1985, 191 (02) :171-175

[5] VITAMIN-D METABOLISM IN HUMAN-PREGNANCY - CONCENTRATIONS OF FREE AND SULFATED 25-HYDROXYVITAMIN-D3 IN MATERNAL AND FETAL PLASMA AT TERM [J].

AXELSON, M ;

CHRISTENSEN, NJ .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1988, 31 (01) :35-39

[6]

Ball G.F. M., 1988, Fat-soluble vitamin assays in food analysis: a comprehensive review. Fat-soluble vitamin assays in food analysis: a comprehensive review

[7]

Ball GFM, 2008, VITAMINS THEIR ROLE, P188

[8] Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS [J].

Ding, Shujing ;

Schoenmakers, Inez ;

Jones, Kerry ;

Koulman, Albert ;

Prentice, Ann ;

Volmer, Dietrich A. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 398 (02) :779-789

[9] Ultrasensitive Quantification of Serum Vitamin D Metabolites Using Selective Solid-Phase Extraction Coupled to Microflow Liquid Chromatography and Isotope-Dilution Mass Spectrometry [J].

Duan, Xiaotao ;

Weinstock-Guttman, Bianca ;

Wang, Hao ;

Bang, Eunjin ;

Li, Jun ;

Ramanathan, Murali ;

Qu, Jun .

ANALYTICAL CHEMISTRY, 2010, 82 (06) :2488-2497

[10]

Gomes FP, 2013, BIOANALYSIS, V5, P3063, DOI [10.4155/BIO.13.283, 10.4155/bio.13.283]

← 1 2 3 4 →