First isolation of West Nile virus from a patient with encephalitis in the United States

被引:86
作者
Huang, CN
Slater, B
Rudd, R
Parchuri, N
Hull, R
Dupuis, M
Hindenburg, A
机构
[1] New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA
[2] Winthrop Univ Hosp, Mineola, NY 11501 USA
关键词
D O I
10.3201/eid0812.020532
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
West Nile virus (WNV) was isolated from a patient who developed encephalitis while undergoing treatment with CHOP (cyclophosphamide, hydroxydoxorubicin, vincristine [Oncovin], predisone) and rituximab for a non-Hodgkin B-cell lymphoma. Both standard reverse transcription-polymerase chain reaction (RT-PCR) and Taqman RT-PCR established the diagnosis of WNV infection from cerebrospinal fluid (CSF). Several whole blood samples and one serum sample underwent further testing. CSF and serum samples were negative for WNV antibody; however, all samples were positive by both RT-PCR assays, Infectious virus was recovered from a blood sample, and its identity was confirmed by using a WNV-specific immunofluorescence assay. The complete WNV genomes determined from CSF and from the virus isolate adapted from cell culture were the same. The results represent the first complete WNV genome sequence obtained directly from human CSF and the first time that infectious WNV has been recovered from a patient with encephalitis in North America.
引用
收藏
页码:1367 / 1371
页数:5
相关论文
共 14 条
  • [1] Identification of a Kunjin/West Nile-like flavivirus in brains of patients with New York encephalitis
    Briese, T
    Jia, XY
    Huang, C
    Grady, LJ
    Lipkin, WI
    [J]. LANCET, 1999, 354 (9186) : 1261 - 1262
  • [2] Detection of West Nile virus sequences in cerebrospinal fluid
    Briese, T
    Glass, WG
    Lipkin, WI
    [J]. LANCET, 2000, 355 (9215) : 1614 - 1615
  • [3] ANTIGENIC RELATIONSHIPS BETWEEN FLAVIVIRUSES AS DETERMINED BY CROSS-NEUTRALIZATION TESTS WITH POLYCLONAL ANTISERA
    CALISHER, CH
    KARABATSOS, N
    DALRYMPLE, JM
    SHOPE, RE
    PORTERFIELD, JS
    WESTAWAY, EG
    BRANDT, WE
    [J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 37 - 43
  • [4] Centers for Disease Control and Prevention (CDC), 1999, MMWR Morb Mortal Wkly Rep, V48, P845
  • [5] CHOP chemotherapy plus rituximab compared with CHOP alone in elderly patients with diffuse large-B-cell lymphoma.
    Coiffier, B
    Lepage, E
    Brière, J
    Herbrecht, R
    Tilly, H
    Bouabdallah, R
    Morel, P
    Van den Neste, E
    Salles, G
    Gaulard, P
    Reyes, F
    Gisselbrecht, C
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (04) : 235 - 242
  • [6] European phase II study of rituximab (chimeric anti-CD20 monoclonal antibody) for patients with newly diagnosed mantle-cell lymphoma and previously treated mantle-cell lymphoma, immunocytoma, and small B-cell lymphocytic lymphoma
    Foran, JM
    Rohatiner, AZS
    Cunningham, D
    Popescu, RA
    Solal-Celigny, P
    Ghielmini, M
    Coiffier, B
    Johnson, PWM
    Gisselbrecht, C
    Reyes, F
    Radford, JA
    Bessell, EM
    Souleau, B
    Benzohra, A
    Lister, TA
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 2000, 18 (02) : 317 - 324
  • [7] Unusual viral infections (progressive multifocal leukoencephalopathy and cytomegalovirus disease) after high-dose chemotherapy with autologous blood stem cell rescue and peritransplantation rituximab
    Goldberg, SL
    Pecora, AL
    Alter, RS
    Kroll, MS
    Rowley, SD
    Waintraub, SE
    Imrit, K
    Preti, RA
    [J]. BLOOD, 2002, 99 (04) : 1486 - 1488
  • [8] HAYES CG, 1989, ARBOVIRUSES EPIDEMIO, V7, P59
  • [9] Hindiyeh M, 2001, EMERG INFECT DIS, V7, P748
  • [10] Genetic analysis of West Nile New York 1999 encephalitis virus
    Jia, XY
    Briese, T
    Jordan, I
    Rambaut, A
    Chi, HC
    Mackenzie, JS
    Hall, RA
    Scherret, J
    Lipkin, WI
    [J]. LANCET, 1999, 354 (9194) : 1971 - 1972