Comparison of the reliability of Gram-negative and Gram-positive flags of the Sysmex UF-5000 with manual Gram stain and urine culture results

被引:15
作者
Enko, Dietmar [1 ,2 ]
Stelzer, Ingeborg [2 ]
Boeckl, Michael [2 ]
Schnedl, Wolfgang J. [3 ]
Meinitzer, Andreas [1 ]
Herrmann, Markus [1 ]
Toetsch, Martin [4 ]
Gehrer, Michael [4 ]
机构
[1] Med Univ Graz, Clin Inst Med & Chem Lab Diagnost, Auenbruggerpl 15, A-8036 Graz, Austria
[2] Gen Hosp Hochsteiermark, Inst Clin Chem & Lab Med, Leoben, Austria
[3] Practice Gen Internal Medicince, Bruck Mur, Austria
[4] Gen Hosp Hochsteiermark, Inst Pathol, Leoben, Austria
关键词
automation; bacteriuria; urinalysis; urinary tract infections; FLOW-CYTOMETRY; SEDIMENT ANALYZER; TRACT-INFECTIONS; PERFORMANCE; DIAGNOSIS; BACTERIA;
D O I
10.1515/cclm-2020-1263
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: Recently, the fully automated flow cytometry-based UF-5000 (Sysmex Corboration, Kobe, Japan) urine sediment analyzer was developed providing bacteria (BACT) info flags for more accurate bacterial discrimination of urinary tract infections (UTIs). This study aimed to compare the reliability of the UF-5000 BACT-info flags with manual Gram stain and urine culture as the gold standard method. Methods: A total of 344 urine samples were analyzed on the UF-5000 and compared with manual microscopic Gram stain and urine cultures. Agreement was assessed by Cohen's kappa (kappa) analysis. The Youden index was used to determine the optimal BACT and white blood cell (WBC) cut-off points for discriminating positive and negative urine cultures. Results: Overall 98/344 (28.5%) samples were urine culture positive at a cut-off of >= 10(5) CFU/mL. "Gram-negative?" UF-5000 BACT-Info flags showed a better concordance of 25/40 (62.5%) with urine culture compared to Gram stain with 30/50 (60%). The results for UF-5000 discrimination of Gram-positive and Gram-negative microorganisms demonstrated a substantial (kappa = 0.78) and fair (kappa = 0.40) agreement with urine culture. Optimal cut-off points detecting positive urine cultures were 135 BACT/mu L (sensitivity [SE]: 92.1%, specificity [SP]: 85.4%, positive predictive value [PPV]: 71%, negative predictive value [NPV]: 96%) and 23 WBC/mu L (SE: 73.5%, SP: 84.1%, PPV: 65%, NPV: 89%). Conclusions: The UF-5000 analyzer (Sysmex) is a reliable diagnostic tool for UTI screening. The displayed BACT-Info flags allow a quick diagnostic orientation for the clinician. However, the authors suggest verifying the automated Gram categories with urine culture.
引用
收藏
页码:619 / 624
页数:6
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