CHARACTERIZATION OF TRYPSIN-LIKE ENZYMES FROM THE MIDGUT OF MORIMUS FUNEREUS (COLEOPTERA: CERAMBYCIDAE) LARVAE

被引：11
作者：
Loncar, N. [1 ]
Bozic, Natasa [2 ]
Nenadovic, Vera [3 ]
Ivanovic, Jelisaveta [3 ]
Vujcic, Z. [1 ]
机构：
[1] Univ Belgrade, Fac Chem, Dept Biochem, Belgrade 11000, Serbia
[2] Univ Belgrade, Inst Chem Technol & Met, Ctr Chem, Belgrade 11000, Serbia
[3] Sinisa Stankovic Inst Biol Res, Dept Insect Physiol & Biochem, Belgrade 11060, Serbia
来源：
ARCHIVES OF BIOLOGICAL SCIENCES | 2009年 / 61卷 / 04期
关键词：
Cerambycidae; Morimus funereus; trypsin; midgut peptidases; synthetic substrate; zymogram; TENEBRIO-MOLITOR COLEOPTERA; DIGESTIVE PROTEINASES; PARTIAL-PURIFICATION; NEUROSECRETION;
D O I：
10.2298/ABS0904713L
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.
引用
收藏
页码：713 / 718
页数：6
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