Bixin regulates mRNA expression involved in adipogenesis and enhances insulin sensitivity in 3T3-L1 adipocytes through PPARγ activation

被引：82

作者：

Takahashi, Nobuyuki ^{[1
]}

Goto, Tsuyoshi ^{[1
]}

Taimatsu, Aki ^{[1
]}

Egawa, Kahori ^{[1
]}

Katoh, Sota ^{[1
]}

Kusudo, Tatsuya ^{[1
]}

Sakamoto, Tomoya ^{[1
]}

Ohyane, Chie ^{[1
]}

Lee, Joo-Young ^{[1
]}

Kim, Young-il ^{[1
]}

Uemura, Taku ^{[1
]}

Hirai, Shizuka ^{[1
]}

Kawada, Teruo ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Lab Mol Funct Food, Uji, Kyoto 6110011, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 04期

关键词：

Bixin; Norbixin; PPAR; Adipocyte; Lipid metabolism; GENE-EXPRESSION; DEHYDROABIETIC ACID; LIPID-PEROXIDATION; NORBIXIN; ALPHA; DIFFERENTIATION; ADIPONECTIN; MACROPHAGES; AURAPTENE; AGONIST;

D O I：

10.1016/j.bbrc.2009.10.162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin resistance is partly due to suppression of insulin-induced glucose uptake into adipocytes. The uptake is dependent on adipocyte differentiation, which is controlled at mRNA transcription level. The peroxisome proliferator-activated receptor (PPAR), a ligand-regulated nuclear receptor, is involved in the differentiation. Many food-derived compounds serve as ligands to activate or inactivate PPAR. In this study, we demonstrated that bixin and norbixin (annatto extracts) activate PPAR gamma by luciferase reporter assay using GAL4-PPAR chimera proteins. To examine the effects of bixin on adipocytes, 3T3-L1 adipocytes were treated with bixin or norbixin. The treatment induced mRNA expression of PPAR gamma target genes such as adipocyte-specific fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and adiponectin in differentiated 3T3-L1 adipocytes and enhanced insulin-dependent glucose uptake. The observations indicate that bixin acts as an agonist of PPAR gamma and enhances insulin sensitivity in 3T3-L1 adipocytes, suggesting that bixin is a valuable food-derived compound as a PPAR ligand to regulate lipid metabolism and to ameliorate metabolic syndrome. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1372 / 1376

页数：5

共 24 条

[1] DNA damage and aberrant crypt foci as putative biomarkers to evaluate the chemopreventive effect of annatto (Bixa orellana L.) in rat colon carcinogenesis [J].