Phenylboronic acid-incorporated elastin-like polypeptide nanoparticle drug delivery systems

被引：20

作者：

Chen, Weizhi ^{[1
,2
]}

Ji, Shilu ^{[1
,2
]}

Qian, Xiaoping ^{[1
,2
]}

Zhang, Yajun ^{[1
,2
]}

Li, Cheng ^{[1
,2
]}

Wu, Wei ^{[1
,2
]}

Wang, Fei ^{[3
]}

Jiang, Xiqun ^{[1
,2
]}

机构：

[1] Nanjing Univ, Coll Chem & Chem Engn, Dept Polymer Sci & Engn, Nanjing 210093, Jiangsu, Peoples R China

[2] Nanjing Univ, Jiangsu Key Lab Nanotechnol, Nanjing 210093, Jiangsu, Peoples R China

[3] Nanjing Forestry Univ, Jiangsu Key Lab Biomass Based Green Fuels & Chem, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China

来源：

POLYMER CHEMISTRY | 2017年 / 8卷 / 13期

关键词：

IN-VIVO EVALUATION; POLYMERIC NANOPARTICLES; DOXORUBICIN DELIVERY; ANTITUMOR-ACTIVITY; PROTEIN; PACLITAXEL; MICELLES; ALBUMIN; PEPTIDE; TRANSFERRIN;

D O I：

10.1039/c7py00330g

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Packaging hydrophobic drugs into nanoparticles can improve their aqueous solubility, tumor-specific accumulation and therapeutic effect. In this study, we prepared phenylboronic acid-incorporated elastin-like polypeptide (ELP) nanoparticles by polymerizing N-3-acrylamidophenylboronic acid (APBA) in the presence of ELP which was expressed from a plasmid with the ELP gene in Escherichia coli. It was found that phenylboronic acid-incorporated elastin-like polypeptide nanoparticles (ELP-PAPBA NPs) had a spherical shape with the size of 100 nm and were highly stable in an aqueous medium with a wide range of pH. The cellular uptake experiment showed that the ELP-PAPBA NPs could be rapidly taken up by single cells and multicellular spheroids (MCs). However, the cellular uptake of ELP-PAPBA NPs decreased dramatically when tumor cells were pretreated with free APBA or free sialic acid, suggesting the interaction between boronic acid-incorporated nanoparticles and overexpressed sialic acid in cancer cells. When loaded with the drug doxorubicin (DOX), the ELP-PAPBA NPs showed a loading content of about 10% and an encapsulation efficiency of about 85%. Bio-distribution and in vivo anticancer efficiency experiments revealed that DOX-loaded ELP-PAPBA NPs had a better tumor accumulation and penetration, lower heart side effects and significant superior anticancer activity in H22 tumor-bearing mice compared to free DOX.

引用

页码：2105 / 2114

页数：10

共 63 条

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