Heterologous expression of the Kluyveromyces marxianus endopolygalacturonase gene (EPG1) using versatile autonomously replicating vector for a wide range of host

被引：23

作者：

Bartkeviciute, D ^{[1
]}

Siekstele, R ^{[1
]}

Sasnauskas, K ^{[1
]}

机构：

[1] Inst Biotechnol, LT-2028 Vilnius, Lithuania

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2000年 / 26卷 / 9-10期

关键词：

Kluyveromyces marxianus endopolygalacturonase; yeast; expression; disruption; vector; stability;

D O I：

10.1016/S0141-0229(00)00155-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A versatile plasmid shuttle vector system pKDU7 was constructed, which is useful for the heterologous gene expression in a wide range of Kluyveromyces and Saccharomyces strains. This cloning vector was constructed using the 1.6-mu m circular plasmid pKD1 of Kluyveromyces drosophilarum, the URA3 gene of K. marxianus as well as the pUC19 sequences. The stability of vector in transformants strongly depends on the integrity of the functionally important elements of pKD1. It was shown by comparison of three recombinant vectors, which possessed the pKD1 sequence inserted in different ways. The efficient transformation and stability maintenance of the vector constructed in various strains of Kluyveromyces and Saccharomyces was shown by the expression of the EPG1 gene of the Kluyveromyces marxianus encoding pectin-degrading endopolygalacturonase. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：653 / 656

页数：4